Invitrogen

ApoA2 Recombinant Rabbit Monoclonal Antibody (43H22L4)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他18个ApoA2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (4)
高级验证 (2)

ApoA2 Antibody in Immunocytochemistry (ICC/IF)

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ApoA2 Antibody (701236) in ICC/IF

Immunofluorescence analysis of Apolipoprotein A-II was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ApoA2 Recombinant Rabbit Monoclonal Antibody (43H22L4) (Product # 701236) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, A... View More

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ApoA2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells treated with PTI (1X for 4hrs) were transfected with Apolipoprotein A-II siRNA and decrease in signal intensity was observed in ICC application using Anti-ApoA2 Recombinant Rabbit Monoclonal Antibody (43H22L4) (Product # 701236). {KD}

产品信息

701236

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

免疫细胞化学 (ICC/IF)

1-5 µg/mL

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

43H22L4

抗原

Peptide corresponding to amino acids 60-71 of human Apo A2

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532435

产品详细信息

This antibody is predicted to react with non-human primate and mouse based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL, and VLDL particles of human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins ( especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease that measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

仅用于科研。不用于诊断过程。未经明确授权不得转售。