Invitrogen

Apolipoprotein D Polyclonal Antibody

Apolipoprotein D Antibody in Immunocytochemistry (ICC/IF)

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Apolipoprotein D Antibody (PA5-27386) in ICC/IF

Apolipoprotein D Polyclonal Antibody detects Apolipoprotein D protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein D protein stained by Apolipoprotein D Polyclonal Antibody (Product # PA5-27386) diluted at 1:500. Blue: Hoechst 33342 staining.

Apolipoprotein D Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Apolipoprotein D Antibody in Western Blot (WB)

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产品信息

PA5-27386

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 189 of Human Apolipoprotein D

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.34 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544862

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2.

Predicted reactivity: Rabbit (81%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Apolipoprotein D (APOD) is a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

LCAT, ApoD, and ApoA1 Expression and Review of Cholesterol Deposition in the Cornea.

Biomolecules

Flores R,Jin X,Chang J,Zhang C,Cogan DG,Schaefer EJ,Kruth HS

PA5-27386 was used in Western Blotting to examine the expression of LCAT by the cell types that comprise from anterior to posterior the three layers of the cornea.