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Aurora B Antibody (MA5-47462) in ICC/IF
Immunofluorescent analysis of Aurora B in HeLa cells. Samples were stained with Aurora B monoclonal antibody (Product # MA5-47462) using a dilution of 1:1,000 (red). Costained with chicken pAb to vimentin dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. The aurora B antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimen... View More
产品信息
MA5-47462
产品规格
种属反应
Dog,
Horse,
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
6G2
抗原
Full length human recombinant aurora B protein expressed in and purified from E. Coli
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A/G
保存液
PBS with 50% glycerol
内含物
5mM sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2942444
靶标信息
The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AIM-1; ARK-2; Aurora 1; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; aurora-B; aurora-related kinase 2; Aurora/IPL1-related kinase 2; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; STK-1
基因别名: AIK2; AIM-1; AIM1; AIRK2; ARK2; AurB; AURKB; aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK1; STK12; STK5
UniProt ID: (Human) Q96GD4
Entrez Gene ID: (Human) 9212, (Dog) 479492, (Horse) 100073050
negative regulation of transcription from RNA polymerase II promoter
negative regulation of B cell apoptotic process
protein phosphorylation
spindle organization
sister chromatid cohesion
aging
cell proliferation
attachment of spindle microtubules to kinetochore
abscission
histone modification
protein sumoylation
anaphase-promoting complex-dependent catabolic process
spindle checkpoint
negative regulation of protein binding
positive regulation of telomere maintenance via telomerase
negative regulation of cytokinesis
positive regulation of cytokinesis
protein localization to kinetochore
cellular response to UV
cleavage furrow formation
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
histone H3-S28 phosphorylation
protein autophosphorylation
mitotic spindle midzone assembly
positive regulation of telomerase activity
regulation of chromosome segregation
regulation of signal transduction by p53 class mediator
positive regulation of telomere capping
