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GenScript

Avi Tag Monoclonal Antibody (1D11D10)

3 篇参考文献

说明书

实验方案

技术支持

货号 A01738-40

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40 µg

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说明书

实验方案

技术支持

抗体检测数据 (5)

Application

Avi Tag Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

Avi Tag Antibody (A01738-40) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of Ai tagged protein transfected CHO cells using Ai Tag Antibody, mAb, Mouse (GenScript, A01738) and Mouse IgG Control (GenScript, A01007). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Avi Tag Antibody in Flow Cytometry (Flow)

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产品信息

A01738-40

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1 µg/mL

免疫细胞化学 (ICC/IF)

1-3 µg/mL

流式细胞分析 (Flow)

1-3 µg 1 x 10^6 cells

酶联免疫吸附实验 (ELISA)

0.02-0.05 µg/mL

其他PubMed (Misc)

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Tag

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

1D11D10

抗原

Avi peptide conjugated to KLH

偶联物

Unconjugated

形式

Lyophilized

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Avi Tag Antibody is a useful tool in analysis of Avi fusion proteins.

靶标信息

The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity.

Cell

Setliff I,Shiakolas AR,Pilewski KA,Murji AA,Mapengo RE,Janowska K,Richardson S,Oosthuysen C,Raju N,Ronsard L,Kanekiyo M,Qin JS,Kramer KJ,Greenplate AR,McDonnell WJ,Graham BS,Connors M,Lingwood D,Acharya P,Morris L,Georgiev IS

B cell receptor (BCR) sequencing is a powerful tool for interrogating immune responses to infection and vaccination, but it provides limited information about the antigen specificity of the sequenced BCRs. Here, we present LIBRA-seq (linking B cell receptor to antigen specificity through sequencing), a technology for high-throughput mapping of paired heavy- and light-chain BCR sequences to their cognate antigen specificities. B cells are mixed with a panel of DNA-barcoded antigens so that both t

Thu Dec 12 00:00:00 UTC 2019

Human antibody-based chemically induced dimerizers for cell therapeutic applications.

Nature chemical biology

Hill ZB,Martinko AJ,Nguyen DP,Wells JA

Chemically induced dimerizers (CIDs) have emerged as one of the most powerful tools for artificially regulating signaling pathways in cells; however currently available CID systems lack the properties desired for use in regulating cellular therapies. Here we report the development of human antibody-based chemically induced dimerizers (AbCIDs) from known small-molecule-protein complexes by selecting for synthetic antibodies that recognize the chemical epitope created by the bound small molecule.

Thu Feb 01 00:00:00 UTC 2018

Characterization of GPCRs in extracellular vesicle (EV).

Methods in cell biology

Medapati MR,Singh A,Korupally RR,Henderson D,Klonisch T,Manda SV,Chelikani P

Extracellular vesicle (EV) are tiny membranous vesicles usually <500nm in size that recently emerged as a new paradigm in human intercellular signaling. EVs have shown a promising role in development of diagnostic markers in many pathophysiological disorders. The presence of chemosensory and therapeutically relevant G protein-coupled receptors (GPCRs) on EV membranes is poorly characterized. Here, we compare different methods including ultracentrifugation and polymer-charge-based separation to i

Thu May 24 00:00:00 UTC 2018

生物信息学

蛋白别名: Avi; BirA