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产品信息
31850
应用
建议稀释比
已发表文章
产品规格
种属反应
Chicken
已发表种属
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified avidin
偶联物
形式
Lyophilized
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
10mM HEPES, pH 7.5, with 0.15M NaCl, 1mg/mL BSA
内含物
0.08% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient
RRID
AB_228209
产品详细信息
Product # 31850 is capable of binding to Avidin D by two mechanisms, either through the antigen binding sites in the Fab portion of the antibody or through multiple biotin residues covalently attached to the protein.
The measured activity of the biotinylated antibody is 99% by a solid phase IgG binding assay.
Reconstitute using 1 mL of distilled water; product may be stored for several weeks as an undiluted liquid. After dilution, do not use for more than one day. After one year, the reconstituted liquid product should be stored frozen in small undiluted aliquots, avoiding freeze/thaw cycles.
靶标信息
Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: avID; Avidin; unnamed protein product
基因别名: AVD
UniProt ID: (Chicken) P02701
Entrez Gene ID: (Chicken) 396260
