Invitrogen

Axl Recombinant Rabbit Monoclonal Antibody (2H29L9)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他56个AXL抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

Axl Antibody in Immunocytochemistry (ICC/IF)

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Axl Antibody (703211) in ICC/IF

Knockdown of Axl was achieved by transfecting T98G cells with Axl specific validated siRNA (Silencer® select Product # s1845). Immunofluorescence analysis was performed on T98G cells (untransfected, panel a-d), transfected with Axl specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed and labelled with Anti-Axl Recombinant Rabbit Monoclonal Antibody (Product # 703211, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27... View More

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Axl Antibody — Antibody specificity was demonstrated by siRNA-mediated knockdown of target protein. T98G cells were transfected with Axl siRNA and decrease in signal intensity was observed in immunofluorescence application using Anti-Axl Recombinant Rabbit Monoclonal Antibody (Product # 703211). {KD}

产品信息

703211

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

2H29L9

抗原

Peptides corresponding to human AXL [1) 28-42 2)745-763]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2734795

产品详细信息

This antibody is predicted to react with Monkey, Horse, Sheep.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。