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B7-H4 Antibody (13-5949-80) in Flow
Staining of non-transfected (left) and human B7-H4 transfected (right) 293T cells with Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.06 µg Anti-Human B7H4 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total cells were used for analysis.
产品信息
13-5949-80
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
H74
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_466806
产品详细信息
Description: The H74 monoclonal antibody was generated and reacts with human B7-S1, also known as B7-H4 and B7x. Cross reactivity of this antibody to other proteins has not been determined. B7S1 is a newly discovered member of the B7 family. It is speculated that the costimulatory regulation of T cells by B7S1 is influenced by the activation status of B cells. While it is reported that BTLA is a counter receptor for B7S1, further studies are needed to definitely determine the B7-H4 ligand. H74 stains human B7-H4 transfected cells and not peripheral blood cells. Exact expression pattern of B7-H4 has not been fully characterized.
Applications Reported: The H74 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This H74 antibody has been tested by flow cytometric analysis of human B7-H4 transfected cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
B7-H4 (B7x, VTCN1) a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response. B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 is expressed primarily on the membrane of lymphoid cells, and as an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, it is involved in mediating cellular and humoral immune responses. Overexpression of B7-H4 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumor cells suppress T cell immunity and facilitate tumor progression. Further, VTCN1 may be a useful biomarker for the early detection of ovarian cancer and Renal Cell Carcinoma (RCC).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7-H4; B7h.5; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; RP11-229A19.4; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; V-set domain-containing T-cell activation inhibitor 1; VTCN1
基因别名: B7-H4; B7h.5; B7H4; B7S1; B7X; PRO1291; UNQ659/PRO1291; VCTN1; VTCN1
UniProt ID: (Human) Q7Z7D3
Entrez Gene ID: (Human) 79679
