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Application
FIGURE: 1 / 4
BACH1 Antibody (PA5-85286) in WB
Western blot analysis of BACH1 in HeLa whole cell and nuclear extracts using BACH1 polyclonal antibody (Product # PA5-85286) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
Product Details
PA5-85286
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human BACH1.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2792429
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Rhesus Monkey (98%).
Positive Control: 293T, HeLa, HeLa nuclear extract.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
BACH1.3 is a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo and/or hetero oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. Some exons of this gene overlap with some exons from the C21orf41 gene, which is transcribed in an opposite orientation to this gene, but which does not seem to encode a protein.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homolog 1; BTB and CNC homology 1, basic leucine zipper transcription factor 1; HA2303; Transcription regulator protein BACH1
Gene Aliases: BACH-1; BACH1; BTBD24
UniProt ID: (Human) O14867
Entrez Gene ID: (Human) 571
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription factor activity, sequence-specific DNA binding
ubiquitin-protein transferase activity
protein binding
heme binding
regulation of transcription involved in G1/S transition of mitotic cell cycle
regulation of transcription involved in G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
DNA repair
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein ubiquitination
positive regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter in response to hypoxia
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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