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BAFF Antibody (500-P163GBT-1MG) in WB
Western Blot: To detect hBAFF by Western Blot analysis BAFF Polyclonal Antibody, Biotin (Product # 500-P163GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P163GBT-1MG
产品规格
已发表种属
Human
宿主/亚型
Goat
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human BAFF
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929578
产品详细信息
AA Sequence of recombinant protein: AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBAFF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BAFF (500-P163G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBAFF.
Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P163GBT-1MG will be provided as 2 x 500 µg
靶标信息
BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
