Invitrogen

BCL6 Monoclonal Antibody (BCL-DWN), APC, eBioscience™

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BCL6 Antibody (17-5453-82) in Flow

Splenocytes isolated from sheep red blood cell-immunized mice were stained with Fixable Viability Dye eFluor® 506 (Product # 65-0866-14) to exclude dead cells from analysis. Then cells were surface stained with Anti-Human/Mouse GL7 PE (Product # 12-5902-82) followed by intracellular staining with 0.015 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.015 µg of Anti-Mouse Bcl-6 APC (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells expressing CD11c, F4/80, Gr-1... View More

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产品信息

17-5453-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.03 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

BCL-DWN

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573214

产品详细信息

Description: This BCL-DWN monoclonal antibody reacts with mouse Bcl-6, which is also known as B-cell lymphoma 6. A member of the POZ family of transcriptional repressors, Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79-kDa protein is essential for germinal center B cell proliferation and survival. Moreover, during B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1.

Applications Reported: This BCL-DWN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This BCL-DWN antibody has been tested by intracellular staining followed by flow cytometric analysis of splenocytes isolated from sheep red blood cell-immunized mice using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Restricted Expression of the Thymoproteasome Is Required for Thymic Selection and Peripheral Homeostasis of CD8⁺ T Cells.

Cell reports

Tomaru U,Konno S,Miyajima S,Kimoto R,Onodera M,Kiuchi S,Murata S,Ishizu A,Kasahara M

17-5453 was used in Flow cytometry/Cell sorting to find that thymoproteasomes are essential for positive selection but that the subsequent change in peptide repertoire in the medulla is also crucial for thymic selection and that β5t-derived peptide must be confined to the thymus to avoid autoimmunity in peripheral tissues.

Tue Jan 15 00:00:00 EST 2019

生物信息学

蛋白别名: B-cell leukemia/lymphoma 5; B-cell leukemia/lymphoma 6; B-cell lymphoma 5 protein (BCL5); B-cell lymphoma 6 protein homolog; cys his2 zinc finger transcription factor; LAZ-3 protein; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc Finger Protein 51 (ZNF51)

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基因别名: Bcl-6; Bcl5; Bcl6

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UniProt ID: (Mouse) P41183

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Entrez Gene ID: (Mouse) 12053

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功能