Invitrogen

BCL6 Monoclonal Antibody (GI191E), eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (4)
高级验证 (2)

BCL6 Antibody in Immunocytochemistry (ICC/IF)

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BCL6 Antibody (14-9887-82) in ICC/IF

Immunofluorescence analysis BCL6 was performed using 70% confluent log phase Raji (B-cell lineage) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with BCL6 Monoclonal Antibody (GI191E) (Product # 14-9887-82) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pr... View More

BCL6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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BCL6 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of BCL6 was observed in Raji, Ramos and Daudi (B-cell lineage) when compared to Jurkat and MOLT-4 (T-cell lineage) using product (Product # 14-9887-82) in western blot. {RE}

产品信息

14-9887-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

GI191E

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10669166

产品详细信息

Description: This GI191E monoclonal antibody reacts with mouse and human Bcl-6, which is also known as B-cell lymphoma 6. A member of the POZ family of transcriptional repressors, Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79-kDa protein is essential for germinal center B cell proliferation and survival. Moreover, during B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1.

Applications Reported: This GI191E antibody has been reported for use in immunoblotting (WB) and immunohistochemistry (FFPE).

Applications Tested: This GI191E antibody has been tested by immunoblotting of reduced cell lysate prepared from A20 cells and by immunohistochemistry on human tissue using high pH antigen retrieval. This antibody can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: B-cell leukemia/lymphoma 5; B-cell leukemia/lymphoma 6; B-cell lymphoma 5 protein; B-cell lymphoma 5 protein (BCL5); B-cell lymphoma 6 protein; B-cell lymphoma 6 protein homolog; B-cell lymphoma 6 protein transcript; BCL-5; BCL-6; cys his2 zinc finger transcription factor; cys-his2 zinc finger transcription factor; LAZ-3 protein; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); lymphoma-associated zinc finger gene on chromosome 3; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc finger protein 51; Zinc Finger Protein 51 (ZNF51); zinc finger transcription factor BCL6S

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基因别名: Bcl-6; BCL5; BCL6; BCL6A; LAZ3; ZBTB27; ZNF51

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UniProt ID: (Human) P41182, (Mouse) P41183

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Entrez Gene ID: (Human) 604, (Mouse) 12053

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