Invitrogen

BCOR Polyclonal Antibody

BCOR Antibody in Immunocytochemistry (ICC/IF)

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BCOR Antibody (PA5-142066) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of BCOR in A431 cells using BCOR Polyclonal Antibody (Product # PA5-142066). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

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产品信息

PA5-142066

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

10 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide with sequence LSATPLYGNVHSW-C (aa 2-14).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2932728

产品详细信息

Additional Information: This antibody is expected to recognise all reported isoforms (NP_060215.4; NP_001116855.1).^M
^M
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.

靶标信息

Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence-specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor. Involved in the repression of TFAP2A; impairs binding of BCL6 and KDM2B to TFAP2A promoter regions. Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem cells; the function implies inhibition of methylation on histone H3 'Lys-4' (H3K4me3) and 'Lys-36' (H3K36me2).

仅用于科研。不用于诊断过程。未经明确授权不得转售。