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BMP9 Polyclonal Antibody

查看其他5个BMP9抗体

BMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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BMP9 Antibody (BS-6909R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BMP9 Polyclonal Antibody, Unconjugated (bs-6909R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructi... View More

BMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BMP9 Antibody in Western Blot (WB)

BMP9 Antibody in Western Blot (WB)

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BMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BMP9 Polyclonal Antibody, Unconjugated (bs-6909R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

BMP9 Antibody in Western Blot (WB) — Lane 1: Mouse Liver lysates; probed with BMP9 Polyclonal Antibody, Unconjugated (bs-6909R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37°C.

BMP9 Antibody in Western Blot (WB) — Lane 1: Human HepG2 lysates probed with BMP9 Polyclonal Antibody, Unconjugated (bs-6909R) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37°C.

产品信息

BS-6909R

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:300

-

免疫组化（石蜡） (IHC (P))

1:400

-

免疫组化（冰冻） (IHC (F))

1:100-1:500

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

1:500-1:1,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide derived from human BMP9, amino acids 351-429.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

内含物

0.02% ProClin 300

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: BMP; BMP-9; Bone morphogenetic protein; Bone morphogenetic protein 9; GDF-2; Growth/differentiation factor 2

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基因别名: BMP-9; BMP9; GDF2; HHT5

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UniProt ID: (Human) Q9UK05, (Mouse) Q9WV56

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Entrez Gene ID: (Human) 2658, (Mouse) 12165, (Rat) 290921

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cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity growth factor

参与通路

ossification angiogenesis patterning of blood vessels vasculogenesis osteoblast differentiation negative regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation cellular iron ion homeostasis negative regulation of DNA replication negative regulation of endothelial cell migration positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of angiogenesis negative regulation of cell growth BMP signaling pathway positive regulation of BMP signaling pathway positive regulation of interleukin-8 production activin receptor signaling pathway growth regulation of apoptotic process regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration positive regulation of endothelial cell differentiation positive regulation of osteoblast differentiation positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell development blood vessel morphogenesis cartilage development pathway-restricted SMAD protein phosphorylation SMAD protein signal transduction positive regulation of cartilage development cellular response to BMP stimulus negative regulation of DNA biosynthetic process glucose metabolic process positive regulation of gene expression regulation of transforming growth factor beta receptor signaling pathway neuron differentiation