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BOB-1 Antibody (PA5-121026) in WB
Western blot analysis of BOB-1 in various lysates. Samples were incubated with BOB-1 Polyclonal antibody (Product # PA5-121026) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-121026
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human POU2AF1 (NP_006226.2).
偶联物
形式
Liquid
浓度
0.92 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914598
产品详细信息
Positive test controls include: HT-29, Mouse spleen, Mouse thymus, Rat spleen. The target is usually found in the following locations: Nucleus.
Immunogen sequence: MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV VLPHQPLATY TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA PWTPYTEYVP HEAVSCPYSA DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTTRSSATPA VGPPLEGPEH QAPLTYFPWP QPLSTLPTST LQYQPPAPAL PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE DSDAYALNHT LSVEGF
靶标信息
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B-cell-specific coactivator OBF-1; BOB-1; BOB1; OCA-B; OCT-binding factor 1; POU domain class 2-associating factor 1; POU domain, class 2, associating factor 1
基因别名: Bob-1; BOB.1; BOB1; OBF-1; OBF.1; OBF1; OCA-B; OCAB; POU2AF1
UniProt ID: (Human) Q16633, (Mouse) Q64693
Entrez Gene ID: (Human) 5450, (Mouse) 18985, (Rat) 690528
