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BRCA1 Polyclonal Antibody

BRCA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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BRCA1 Antibody (BS-0803R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BRCA1 Polyclonal Antibody, Unconjugated (bs-0803R) at 1:400 overnight at 4°C, DAB staining.

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产品信息

BS-0803R

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:400

免疫组化（冰冻） (IHC (F))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

1:20-1:100

酶联免疫吸附实验 (ELISA)

1:500-1:1,000

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide derived from mouse BRCA1, amino acids 64-160.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

内含物

0.02% ProClin 300

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: brca 1; brca-1; breast cancer 1 early onset; breast cancer associated 1; Breast cancer type 1 susceptibility protein homolog; BROVCA12; Delta772-3095; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; RING-type E3 ubiquitin transferase BRCA1

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基因别名: Brca1

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UniProt ID: (Mouse) P48754, (Rat) O54952

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Entrez Gene ID: (Mouse) 12189, (Rat) 497672

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功能