Invitrogen

BRCA2 Monoclonal Antibody (234403)

BRCA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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BRCA2 Antibody (MA5-23942) in IHC (P)

BRCA2 in Human Breast Cancer Tissue. BRCA2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using BRCA2 Monoclonal Antibody (Product # MA5-23942) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG HRP Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval. Tissue was stained u... View More

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产品信息

MA5-23942

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2 µg/mL

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免疫组化（石蜡） (IHC (P))

5-25 µg/mL

免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

234403

抗原

E. coli-derived recombinant human BRCA2 Met1-Thr200

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2608610

产品详细信息

This antibody detects endogenous human BRCA2.

Reconstitute at 0.5 mg/mL in sterile PBS.

靶标信息

Breast Cancer Type 2 susceptibility protein (BRCA2) is a tumor-suppressor protein reported to be involved in double-strand break repair, homologous recombination and S-phase checkpoint activation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Olaparib-Resistant BRCA2^MUT Ovarian Cancer Cells with Restored BRCA2 Abrogate Olaparib-Induced DNA Damage and G2/M Arrest Controlled by the ATR/CHK1 Pathway for Survival.

Cells

Biegała Ł,Gajek A,Marczak A,Rogalska A

MA5-23942 was used in Western Blotting to elucidate olaparib resistance and enhance the efficacy of olaparib and examine intracellular factors exploited by OC cells to achieve decreased sensitivity to PARPi.

Wed Mar 29 00:00:00 EDT 2023

Olaparib-Resistant BRCA2^MUT Ovarian Cancer Cells with Restored BRCA2 Abrogate Olaparib-Induced DNA Damage and G2/M Arrest Controlled by the ATR/CHK1 Pathway for Survival.

Cells

Biegała Ł,Gajek A,Marczak A,Rogalska A

Wed Mar 29 00:00:00 EDT 2023

Loss of MAP3K7 Sensitizes Prostate Cancer Cells to CDK1/2 Inhibition and DNA Damage by Disrupting Homologous Recombination.

Molecular cancer research : MCR

Washino S,Rider LC,Romero L,Jillson LK,Affandi T,Ohm AM,Lam ET,Reyland ME,Costello JC,Cramer SD

MA5-23942 was used in Immunocytochemistry-immunoflourescence to demonstrate that loss of MAP3K7 is a main contributing factor to drug response through disruption of HR in prostate cancer.

Tue Oct 01 00:00:00 EDT 2019

生物信息学

蛋白别名: brca 2; brca-2; BRCA1/BRCA2-containing complex, subunit 2; breast and ovarian cancer susceptibility gene, early onset; breast and ovarian cancer susceptibility protein 2; breast cancer 2 tumor suppressor; breast cancer 2, early onset; Breast cancer type 2 susceptibility protein; FANCB; Fanconi anemia group D1 protein; mutant BRCA2; RP11-298P3.4

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基因别名: BRCA2; BRCC2; BROVCA2; FACD; FAD; FAD1; FANCD; FANCD1; GLM3; PNCA2; XRCC11

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UniProt ID: (Human) P51587

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Entrez Gene ID: (Human) 675

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功能

protease binding single-stranded DNA binding histone acetyltransferase activity protein binding H3 histone acetyltransferase activity H4 histone acetyltransferase activity gamma-tubulin binding damaged DNA-binding protein

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