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BRG1 Antibody (PA5-17003) in ICC/IF
Immunofluorescence analysis of BRG1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BRG1 Polyclonal Antibody (Product # PA5-17003) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilut... View More
产品信息
PA5-17003
产品规格
种属反应
Human,
Mouse,
Non-human primate
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to amino acids surrounding Ala52 of human Brg1
偶联物
形式
Liquid
浓度
31 µg/mL
纯化类型
Antigen affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10983343
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATP-dependent helicase SMARCA4; BAF190A; brahma protein-like 1; BRG1-associated factor 190A; BRM/SWI2-related gene 1; FLJ39786; global transcription activator homologous sequence; homeotic gene regulator; Mitotic growth and transcription activator; nuclear protein GRB1; Protein brahma homolog 1; Protein BRG-1; SNF2-beta; SNF2-like 4; sucrose nonfermenting-like 4; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; Transcription activator BRG1
基因别名: b2b508.1Clo; b2b692Clo; BAF190; BAF190A; BRG1; CSS4; HP1-BP72; hSNF2b; MRD16; RTPS2; SMARCA4; SNF2; SNF2B; SNF2beta; SNF2L4; SNF2LB; SW1/SNF; SWI2
UniProt ID: (Human) P51532, (Mouse) Q3TKT4
Entrez Gene ID: (Human) 6597, (Mouse) 20586
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription coactivator activity
RNA polymerase I CORE element sequence-specific DNA binding
p53 binding
transcription coactivator activity
transcription corepressor activity
helicase activity
protein binding
ATP binding
DNA-dependent ATPase activity
transcription factor binding
Tat protein binding
nucleosomal DNA binding
protein N-terminus binding
androgen receptor binding
DNA polymerase binding
lysine-acetylated histone binding
nucleotide binding
RNA polymerase II regulatory region sequence-specific DNA binding
DNA binding
chromatin binding
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
ATPase activity
protein complex binding
histone binding
DNA helicase
negative regulation of transcription from RNA polymerase II promoter
neural retina development
nucleosome disassembly
chromatin remodeling
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of transcription from RNA polymerase II promoter during mitosis
spermatid development
nervous system development
covalent chromatin modification
positive regulation of Wnt signaling pathway
negative regulation of cell growth
ATP-dependent chromatin remodeling
positive regulation by host of viral transcription
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
negative regulation of androgen receptor signaling pathway
positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
positive regulation of glucose mediated signaling pathway
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
beta-catenin-TCF complex assembly
negative regulation of G1/S transition of mitotic cell cycle
cell morphogenesis
blood vessel development
vasculogenesis
in utero embryonic development
blastocyst growth
blastocyst hatching
liver development
outflow tract morphogenesis
nucleosome assembly
methylation-dependent chromatin silencing
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
glial cell fate determination
heart development
positive regulation of cell proliferation
DNA methylation on cytosine within a CG sequence
chromatin modification
stem cell population maintenance
neurogenesis
extracellular matrix organization
keratinocyte differentiation
regulation of cell migration
forebrain development
hindbrain development
embryonic hindlimb morphogenesis
aortic smooth muscle cell differentiation
negative regulation of apoptotic process
positive regulation of DNA binding
histone H3 acetylation
positive regulation of cell differentiation
embryonic organ morphogenesis
epidermis morphogenesis
definitive erythrocyte differentiation
heart trabecula formation
pharyngeal arch artery morphogenesis
lens fiber cell development
