Invitrogen

BST-2 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

查看其他45个BST-2 (CD317)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (6)
高级验证 (2)

BST-2 Antibody in Immunocytochemistry (ICC/IF)

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BST-2 Antibody (PA5-80385) in ICC/IF

Immunofluorescence analysis of Bone marrow stromal antigen 2 was performed using 70% confluent log phase HeLa cells treated with 1 ng/mL IFNa for 24 Hrs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BST-2 Polyclonal Antibody (Product # PA5-80385) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed... View More

BST-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BST-2 Antibody in Immunoprecipitation (IP)

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BST-2 Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis using BST-2 Polyclonal Antibody (Product # PA5-80385), shows upregulation upon treatment with IFNa in HeLa cells. {TM}

产品信息

PA5-80385

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:2,000-1:10,000

免疫细胞化学 (ICC/IF)

1:200

酶联免疫吸附实验 (ELISA)

1:25,000-1:50,000

免疫沉淀 (IP)

1-2 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human BST2 Protein (Asn49-Ser160)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2787707

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human BST2.

靶标信息

BST2 (CD317, HM1.24 antigen, DAMP-2, tetherin) is an integral membrane protein that is involved in the development and growth of B-cells. The surface expression of BST2 on fibroblast cell lines facilitated the stromal cell-dependent growth of DW34, a pre-B-cell line. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, NK cells and DCs (at protein level), and myeloma cells. Research studies have shown that BST2 also functions as an inhibitor of retrovirus release from human cells whose activity is antagonized by the HIV-1 accessory protein, Vpu. While BST2 causes retention of virions on cell surfaces or endocytosis into BST2-positive compartments, its depletion abolished the viral requirement for Vpu for virus release, an activity that may represent a potential therapeutic strategy for the treatment of HIV/AIDS. Another disease associated with BST2 dysfunction is stomatitis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。