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Bestrophin 1 Antibody (PA5-78867) in WB
Western blot analysis of Bestrophin 1 in Lane 1: human A549 whole cell lysate, Lane 2: human SGC-7901 whole cell lysate, Lane 3: human U20S whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with Bestrophin 1 polyclonal antibody (Product # PA5-78867)... View More
产品信息
PA5-78867
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence of human Bestrophin (RFIYRLALTEEQQLMFEKLTLYCD).
偶联物
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2745983
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
The retinal pigment epithelium (RPE) and choroid represent a differentiated system of the eye that sustains normal retinal health and function. Best vitelliform macular dystrophy, known as Best disease, is an early-onset autosomal dominant condition in which accumulation of lipofuscin-like material within and beneath the RPE leads to progressive loss of central vision. The lipofuscin-like material in the macular area appears as a yellow mass like the yolk of an egg that later becomes darker and irregular in color, a process known as "scrambling the egg". Best disease is frequently a reflection of mutations in the Bestrophin gene, which encodes a protein containing four putative transmembrane domains and localizes to the basolateral plasma membrane of RPE cells. Human Bestrophin forms oligomeric chloride channels that are sensitive to intracellular calcium. Missense mutations at the Bestrophin locus reduces or abolishes Bestrophin protein mediated membrane current. The human Bestrophin gene encodes a 585 amino acid protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: BEST 1; Best disease; Best1V1Delta2; Bestrophin-1; Bestrophin1; TU15B; Vitelliform macular dystrophy protein 2; VMD 2
基因别名: ARB; BEST; BEST1; BMD; RP50; TU15B; VMD2
UniProt ID: (Human) O76090
Entrez Gene ID: (Human) 7439
