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Biotin Antibody (MA5-55397) in WB
Western Blot analysis of various lysates using Biotin Recombinant Monoclonal Antibody (Product # MA5-55397) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
产品信息
MA5-55397
产品规格
种属反应
Chemical
宿主/亚型
Rabbit
/ IgG1
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
0E9F4
抗原
Biotin
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Dry ice (international)
靶标信息
Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Vitamin B4
