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Mouse IgG (H+L) Secondary Antibody (SA1-9507) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1) and K-562 (Lane 2). The blots were probed with Anti SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Bovine anti-Mouse IgG Secondary Antibody, Biotin (Product # SA1-9507) at dilutions 1:50,000 (Fig. 1), 1:100,000 (Fig. 2) and 1:200,000 (Fig. 3). A 22 kDa band corres... View More
产品信息
SA1-9507
应用
建议稀释比
免疫印迹 (WB)
1:5,000-1:20,000
酶联免疫吸附实验 (ELISA)
1:1000-1:4000
产品规格
种属反应
Mouse
宿主/亚型
Bovine
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Immunoglobulin isolated from mouse serum.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1075986
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
This antibody reacts with the heavy and light chains of the immunoglobulin molecule.
SA1-9507 has been successfully used in Western blot and ELISA applications.
SA1-9507 immunogen is immunoglobulin isolated from mouse serum.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
