Invitrogen

Brachyury Monoclonal Antibody (07)

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抗体检测数据 (1)

Brachyury Antibody in Immunocytochemistry (ICC/IF)

Brachyury Antibody in Immunocytochemistry (ICC/IF)

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Brachyury Antibody (MA5-43052) in ICC/IF

Immunocytochemistry analysis of Brachyury in HeLa cells. Samples were treated with 4% PFA, permeabilized with 1% Triton X-100 in PBS, and blocked with 10% serum, incubated in Brachyury monoclonal antibody (Product # MA5-43052) at a dilution of 1:60, followed by Alexa Fluor488-conjugated Goat Anti-mouse IgG.

Brachyury Antibody in Immunocytochemistry (ICC/IF)

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Brachyury Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Brachyury in HeLa cells. Samples were treated with 4% PFA, permeabilized with 1% Triton X-100 in PBS, and blocked with 10% serum, incubated in Brachyury monoclonal antibody (Product # MA5-43052) at a dilution of 1:60, followed by Alexa Fluor488-conjugated Goat Anti-mouse IgG.

产品信息

MA5-43052

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:20-1:100

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

07

抗原

A synthetic peptide corresponding to the C-terminus of the Human Brachyury/TBXT

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2912192

产品详细信息

Specificity: Human Brachyury/TBXT

靶标信息

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Brachyury protein; Protein T; RP11-459F1.1; T brachyury homolog; T, brachyury homolog; T-box transcription factor T

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基因别名: SAVA; T; TBXT; TFT

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UniProt ID: (Human) O15178

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Entrez Gene ID: (Human) 6862

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RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Rel homology transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter vasculogenesis somitogenesis neural plate morphogenesis neural tube closure heart morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription, DNA-templated signal transduction penetration of zona pellucida mesoderm development mesoderm migration involved in gastrulation positive regulation of cell proliferation anterior/posterior axis specification, embryo notochord formation signal transduction involved in regulation of gene expression BMP signaling pathway post-anal tail morphogenesis embryonic skeletal system development canonical Wnt signaling pathway bone morphogenesis SMAD protein signal transduction determination of heart left/right asymmetry cellular response to retinoic acid primitive streak formation