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BrdU Antibody (MA3-071-D488) in Flow
Flow cytometry analysis of BrdU incorporation in actively cycling Ramos cells. For in vitro labeling of cells, 20 µL of a 1 mM BrdU/PBS solution was added to 2 mL cell suspension containing 2x10^6 Ramos cells and incubated for 2 hours at 37C. Formaldehyde fixed cells were washed and permeabilized. To expose DNA incorporated BrdU, cells were treated with DNase for 1 hour at 37C. Actively cycling Ramos cells were stained with a BrdU-DyLight 488 conjugated monoclonal antibody (Product # MA3-071-D488, red histogram) at a 1:100 dilution for 30 ... View More
产品信息
MA3-071-D488
产品规格
种属反应
Chemical
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
BU-1
抗原
5-iodouridine covalently coupled to ovalbumin
偶联物
DyLight™ 488
激发/发射光谱
492/519 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with proprietary stabilizer
内含物
0.02% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2537629
产品详细信息
Unconjugated MA3-071 has been successfully used in immunofluorescence, immunohistochemistry, and flow cytometry applications. DyLight 488 conjugated MA3-071-D488 has been successfully used in flow cytometry applications.
靶标信息
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
