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BrdU Antibody (B35133) in IHC
Disease models & mechanisms 2017 -
Fig. 5. Effects of doxorubicin and epirubicin on proliferation and apoptosis in the hyperplastic livers of transgenic zebrafish larvae. (A) An example of the whole liver after dissection in both bright-field and fluorescent images. Scale bars: 50 mum. (B) Representative images of BrdU and TUNEL staining in the DMSO-, doxorubicin- and epirubicin-treated zebrafish liver with EGFP-kras G12V over-expression. Sc... View More
产品信息
B35133
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
已发表种属
Chemical
宿主/亚型
Mouse
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
MoBU-1
抗原
BrdU
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
5mM sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536437
产品详细信息
Cell proliferation can be measured with BrdU following its incorporation into newly synthesized DNA and its subsequent detection with an anti-BrdU antibody. Although this method is rapidly being replaced with the recently developed click chemistry-based Click-iTTM EdU assay, an advantage of the antibody clone MoBU-1 is that it does not cross-react with the thymidine analog EdU (5-ethynyl-2'-deoxyuridine), thus making it valuable for use in dual-pulse applications. Traditionally, the dual-pulse method, which is used for studying biological events such as neurogenesis and cell differentiation or evaluating anti-cancer drug efficacy, employs BrdU immunocytochemistry with iododeoxyuridine (IdU) or chlorodeoxyuridine (CldU), using multiple antibodies for detection. However, using sequential pulses of the thymidine analogs EdU and BrdU greatly simplifies dual-pulse labeling, and allows a straightforward and reliable method of distinguishing BrdU- and EdU-labeled cells. In addition to no cross-reactivity with EdU, the MoBU-1 clone has a high specificity for BrdU. This clone can also readily detect bromouridine (BrU) incorporated into RNA or BrdUTP incorporated into DNA strand breaks via the TUNEL assay. Samples labeled with the Alexa FluorTM conjugates of MoBU-1 can be directly imaged by fluorescence microscopy or high-content imaging. The approximate fluorescence excitation/emission maxima for Alexa FluorTM647 conjugate are 650/665 or 670 in nm.
Detection with BrdU antibodies requires not only fixed and permeabilized cells, but also denatured DNA for the BrdU antibody to gain access to the incorporated BrdU. The appropriate dilution of the antibody and the incubation time should be determined empirically. We recommend the following starting points for Alexa FluorTM conjugate: 1-4 µg/mL (1:200-1:50 dilution). Labeled samples are compatible with all slide preparation methods, including wet mount and prepared mounting media (i.e., SlowFadeTM Antifade and ProLongTM Gold Kits).
靶标信息
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
