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Brevican Antibody (MA5-45598) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Brevican monoclonal antibody (Product # MA5-45598) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red... View More
产品信息
MA5-45598
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
N294A/6
抗原
Fusion protein amino acids 219-655 of rat Brevican
偶联物
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932052
产品详细信息
1 µg/mL of MA5-45598 was sufficient for detection of Brevican in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 140kDa (and smaller due to proteolytic cleavage).
This antibody was formerly sold as clone S294A-6.
靶标信息
Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BEHAB; Brain-enriched hyaluronan-binding protein; brevican (brain specific proteoglycan in the aggrecan family); Brevican core protein; Chondroitin sulfate proteoglycan 7; chondroitin sulfate proteoglycan BEHAB
基因别名: ALPBRE; BCAN; BEHAB; CSPG7; UNQ2525/PRO6018
UniProt ID: (Human) Q96GW7, (Rat) P55068, (Mouse) Q61361
Entrez Gene ID: (Human) 63827, (Rat) 25393, (Mouse) 12032
skeletal system development
cell adhesion
central nervous system development
hippocampus development
extracellular matrix disassembly
extracellular matrix organization
glycosaminoglycan metabolic process
chondroitin sulfate biosynthetic process
chondroitin sulfate catabolic process
dermatan sulfate biosynthetic process
regulation of neuronal synaptic plasticity
