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NeoBiotechnologies
Bromodeoxyuridine (BrdU) Recombinant Mouse Monoclonal Antibody (rBRD.3)
Application
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Bromodeoxyuridine (BrdU) Antibody (MSM11-3464-P1) in SDS-PAGE
SDS-PAGE Analyis Purified Bromodeoxyuridine (BrdU) Recombinant Monoclonal Antibody (Product # MSM11-3464-P1). Confirmation of Purity and Integrity of Antibody.
产品信息
MSM11-3464-P1
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
rBRD.3
抗原
Bromodeoxyuridine (BrdU) conjugated to BSA
偶联物
Unconjugated
形式
Liquid
浓度
200 µg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4, with 0.05% BSA
内含物
0.05% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
靶标信息
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
