Invitrogen

Bub3 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他13个Bub3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

Bub3 Antibody in Immunocytochemistry (ICC/IF)

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Bub3 Antibody (PA5-17071) in ICC/IF

Immunofluorescence analysis of Bub3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Bub3 Polyclonal Antibody (Product # PA5-17071) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034)... View More

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Bub3 Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. HeLa cells were transfected with Bub3 siRNA and reduction of signal was observed in Western Blot using Bub3 Polyclonal Antibody (Product # PA5-17071). {KD}

产品信息

PA5-17071

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Hamster, Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to carboxy-terminal residues of human Bub3

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10987257

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3 mitotic checkpoint protein; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; mitotic checkpoint component; Mitotic checkpoint protein BUB3; OTTHUMP00000020682; testicular tissue protein Li 27; WD repeat type I transmembrane protein A72.5

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基因别名: Aa2-050; AU019800; AU021329; AU043350; AW146323; BUB3; BUB3L; C78067; hBUB3

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UniProt ID: (Human) O43684, (Mouse) Q9WVA3