Bioss

CARM1 Polyclonal Antibody

查看其他16个PRMT4抗体

抗体检测数据 (5)

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CARM1 Antibody (BS-4098R) in IHC (P)

Paraformaldehyde-fixed, paraffin-embedded Rat Adrenal Gland tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CARM1 Polyclonal Antibody, Unconjugated (bs-4098R) at 1:400 overnight at 4°C, followed by secondary and DAB staining.

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CARM1 Antibody in Western Blot (WB)

CARM1 Antibody in Western Blot (WB)

Product Image Gallery

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin-embedded Rat Adrenal Gland tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CARM1 Polyclonal Antibody, Unconjugated (bs-4098R) at 1:400 overnight at 4°C, followed by secondary and DAB staining.

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin-embedded Mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CARM1 Polyclonal Antibody, Unconjugated (bs-4098R) at 1:400 overnight at 4°C, followed by secondary and DAB staining.

CARM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti-CARM1 Polyclonal Antibody, Unconjugated (bs-4098R) at 1:200 followed by conjugation to the secondary antibody.

CARM1 Antibody in Western Blot (WB) — Lane 1: NIH/3T3 cell lysates; Lane 2: DU145 cell lysates probed with CARM1 Polyclonal Antibody, Unconjugated (bs-4098R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

CARM1 Antibody in Western Blot (WB) — NIH/3T3 cell lysates probed with CARM1 Polyclonal Antibody, Unconjugated (bs-4098R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

产品信息

BS-4098R

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化（石蜡） (IHC (P))

1:400

-

免疫组化（冰冻） (IHC (F))

1:100-1:500

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

1:500-1:1,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide derived from human CARM1, amino acids 411-510.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

内含物

0.02% ProClin 300

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Coactivator-associated arginine methyltransferase 1; coactivator-associated arginine methyltransferase 1 variant 5; Histone-arginine methyltransferase CARM1; protein arginine methyltransferase; Protein arginine N-methyltransferase 4

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基因别名: CARM1; PRMT4

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UniProt ID: (Human) Q86X55, (Mouse) Q9WVG6, (Rat) Q4AE70

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Entrez Gene ID: (Human) 10498, (Mouse) 59035, (Rat) 363026

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transcription coactivator activity protein binding beta-catenin binding protein methyltransferase activity histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity ligand-dependent nuclear receptor transcription coactivator activity protein-arginine omega-N asymmetric methyltransferase activity histone methyltransferase activity (H3-R17 specific) histone methyltransferase activity protein homodimerization activity transcription regulatory region DNA binding lysine-acetylated histone binding rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity protein modifying enzyme

参与通路

regulation of growth plate cartilage chondrocyte proliferation transcription, DNA-templated regulation of transcription, DNA-templated DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest aging positive regulation of cell proliferation viral process histone methylation peptidyl-arginine methylation, to asymmetrical-dimethyl arginine intracellular estrogen receptor signaling pathway negative regulation of protein binding regulation of intracellular estrogen receptor signaling pathway histone H3-R2 methylation histone H3-R17 methylation cellular lipid metabolic process positive regulation of fat cell differentiation response to cAMP endochondral bone morphogenesis regulation of mRNA binding negative regulation of dendrite development protein methylation pathogenesis chromatin modification intracellular steroid hormone receptor signaling pathway methylation histone arginine methylation peptidyl-arginine N-methylation mRNA processing RNA splicing