Invitrogen

CBP Polyclonal Antibody

CBP Antibody in Immunocytochemistry (ICC/IF)

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CBP Antibody (PA1-847) in ICC/IF

Immunofluorescent analysis of CREB Binding Protein (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB Binding Protein polyclonal antibody (Product # PA1-847) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incub... View More

CBP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CBP Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-847

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看8篇已发表文章 8篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:1,000-1:5,000

免疫细胞化学 (ICC/IF)

1:50-1:500

查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀实验 (ChIP)

1-3 µL

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2083939

产品详细信息

PA1-847 detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) from human and mouse tissues and cells.

PA1-847 has been successfully used in ChIP, ICC/IF, Western blot and IHC-P procedures. By Western blot, this antibody detects an ~265 kDa protein representing CBP and a larger, unknown protein at ~400 kDa from HeLa cell lysate.

The PA1-847 immunogen is a synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP. The PA1-847 immunizing peptide (Cat. # PEP-052) is available for use in neutralization and control experiments.

靶标信息

CBP for CREB-binding protein is a 265 kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Purine-Induced IFN-γ Promotes Uric Acid Production by Upregulating Xanthine Oxidoreductase Expression.

Frontiers in immunology

Wang H,Xie L,Song X,Wang J,Li X,Lin Z,Su T,Liang B,Huang D

PA1-847 was used in ChIP assay to explore whether exogenous purines are responsible for increased xanthine oxidoreductase expression and activity.

Thu Mar 03 00:00:00 EST 2022

Purine-Induced IFN-γ Promotes Uric Acid Production by Upregulating Xanthine Oxidoreductase Expression.

Frontiers in immunology

Wang H,Xie L,Song X,Wang J,Li X,Lin Z,Su T,Liang B,Huang D

PA1-847 was used in ChIP assay to explore whether exogenous purines are responsible for increased xanthine oxidoreductase expression and activity.

Thu Mar 03 00:00:00 EST 2022

Convergent organization of aberrant MYB complex controls oncogenic gene expression in acute myeloid leukemia.

eLife

Takao S,Forbes L,Uni M,Cheng S,Pineda JMB,Tarumoto Y,Cifani P,Minuesa G,Chen C,Kharas MG,Bradley RK,Vakoc CR,Koche RP,Kentsis A

PA1-847 was used in Western Blot to conclude that aberrant assembly and sequestration of MYB:CBP/P300 complexes provide a unifying mechanism of oncogenic gene expression in AML.

Tue Feb 02 00:00:00 EST 2021

Convergent organization of aberrant MYB complex controls oncogenic gene expression in acute myeloid leukemia.

eLife

Takao S,Forbes L,Uni M,Cheng S,Pineda JMB,Tarumoto Y,Cifani P,Minuesa G,Chen C,Kharas MG,Bradley RK,Vakoc CR,Koche RP,Kentsis A

PA1-847 was used in Western Blot to conclude that aberrant assembly and sequestration of MYB:CBP/P300 complexes provide a unifying mechanism of oncogenic gene expression in AML.

Tue Feb 02 00:00:00 EST 2021

LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription.

Cell death & disease

Xie X,Lin J,Fan X,Zhong Y,Chen Y,Liu K,Ren Y,Chen X,Lai D,Li X,Li Z,Tang A

Published figure using CBP polyclonal antibody (Product # PA1-847) in Western Blot

Fri Feb 19 00:00:00 EST 2021

Aberrant activation of CYR61 enhancers in colorectal cancer development.

Journal of experimental & clinical cancer research : CR

Xie L,Song X,Lin H,Chen Z,Li Q,Guo T,Xu T,Su T,Xu M,Chang X,Wang LK,Liang B,Huang D

PA1-847 was used in Western Blotting to study the mechanism of action of cysteine-rich 61 in the regulation of transcription in colorectal cancer.

Wed May 22 00:00:00 EDT 2019

Peptidomimetic blockade of MYB in acute myeloid leukemia.

Nature communications

Ramaswamy K,Forbes L,Minuesa G,Gindin T,Brown F,Kharas MG,Krivtsov AV,Armstrong SA,Still E,de Stanchina E,Knoechel B,Koche R,Kentsis A

Published figure using CBP polyclonal antibody (Product # PA1-847) in Western Blot

Tue Jan 09 00:00:00 EST 2018

Expression of glial CBP in steroid mediated neuroprotection in male and female zebra finches.

Journal of chemical neuroanatomy

Klores M,Moon JT,Duncan KA

PA1847 was used in immunohistochemistry to explore the role of cAMP response element-binding protein-binding protein in the estrogen and immune response to injury

Sun Jan 01 00:00:00 EST 2017

Research resource: identification of novel coregulators specific for thyroid hormone receptor-β2.

Molecular endocrinology (Baltimore, Md.)

Hahm JB,Privalsky ML

PA1-847 was used in Western Blotting to identify a series of previously unrecognized proteins that selectively interact with thyroid hormone receptorΒ2, compared with the more widely expressed TRΒ1.

Wed May 01 00:00:00 EDT 2013

Cells in behaviourally relevant brain regions coexpress nuclear receptor coactivators and ovarian steroid receptors.

Journal of neuroendocrinology

Tetel MJ,Siegal NK,Murphy SD

PA1-847 was used in immunohistochemistry and western blot to investigate the expression of nuclear receptor coactivators and ovarian steroid receptors in particular brain regions

Sun Apr 01 00:00:00 EDT 2007

Cells in behaviourally relevant brain regions coexpress nuclear receptor coactivators and ovarian steroid receptors.

Journal of neuroendocrinology

Tetel MJ,Siegal NK,Murphy SD

PA1-847 was used in immunohistochemistry and western blot to investigate the expression of nuclear receptor coactivators and ovarian steroid receptors in particular brain regions

Sun Apr 01 00:00:00 EDT 2007

Nuclear receptor coactivators function in estrogen receptor- and progestin receptor-dependent aspects of sexual behavior in female rats.

Hormones and behavior

Molenda-Figueira HA,Williams CA,Griffin AL,Rutledge EM,Blaustein JD,Tetel MJ

PA1-847 was used in western blot to study the ER and PR action regulated by SRC-1 and CBP in the rat brain

Fri Sep 01 00:00:00 EDT 2006

Nuclear receptor coactivators modulate hormone-dependent gene expression in brain and female reproductive behavior in rats.

Endocrinology

Molenda HA,Griffin AL,Auger AP,McCarthy MM,Tetel MJ

PA1-847 was used in immunocytochemistry to demonstrate the regulatory role of nuclear receptors in ER transcriptional activity in brain and the expression of hormone-dependent behavior.

Fri Feb 01 00:00:00 EST 2002

The transcriptional coactivators p300 and CBP are histone acetyltransferases.

Cell

Ogryzko VV,Schiltz RL,Russanova V,Howard BH,Nakatani Y

PA1-847 was used in western blot to investigate the functional properties of CREB binding protein and p300

Fri Nov 29 00:00:00 EST 1996

生物信息学

蛋白别名: CREB-binding protein; Histone lysine acetyltransferase CREBBP; Protein lactyltransferas CREBBP; Protein-lysine acetyltransferase CREBBP

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基因别名: AW558298; CBP; CBP/p300; CREBBP; KAT3A; p300/CBP; RSTS

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UniProt ID: (Human) Q92793

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Entrez Gene ID: (Human) 1387, (Mouse) 12914

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功能

core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity transcriptional repressor activity, RNA polymerase II transcription factor binding p53 binding chromatin binding damaged DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity histone acetyltransferase activity signal transducer activity protein binding transcription factor binding zinc ion binding acetyltransferase activity peptide N-acetyltransferase activity peroxisome proliferator activated receptor binding MRF binding SMAD binding RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription cofactor activity transferase activity transferase activity, transferring acyl groups activating transcription factor binding metal ion binding histone modifying enzyme

参与通路

negative regulation of transcription from RNA polymerase II promoter response to hypoxia stimulatory C-type lectin receptor signaling pathway regulation of transcription, DNA-templated transcription initiation from RNA polymerase II promoter protein complex assembly protein acetylation signal transduction Notch signaling pathway cell proliferation regulation of smoothened signaling pathway viral process histone acetylation N-terminal peptidyl-lysine acetylation positive regulation of type I interferon production cellular response to UV homeostatic process embryonic digit morphogenesis regulation of apoptotic process cellular lipid metabolic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process positive regulation of cell adhesion molecule production regulation of transcription from RNA polymerase II promoter in response to hypoxia response to interleukin-1 cell-cell adhesion regulation of cellular response to heat positive regulation of G1/S transition of mitotic cell cycle positive regulation of NIK/NF-kappaB signaling beta-catenin-TCF complex assembly transcription, DNA-templated transcription from RNA polymerase II promoter germ-line stem cell population maintenance positive regulation of sequence-specific DNA binding transcription factor activity

CBP Polyclonal Antibody

CBP Antibody (PA1-847) in ICC/IF

产品信息

PA1-847

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

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CBP Polyclonal Antibody

CBP Antibody (PA1-847) in ICC/IF

产品信息

PA1-847

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)