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Application
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CCL28 Antibody (500-P297-1MG) in WB
Western Blot: To detect hMEC by Western Blot analysis CCL28 Polyclonal Antibody (Product # 500-P297-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P297-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MEC (CCL28)
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930285
Product Specific Information
AA Sequence of recombinant protein: SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MEC (CCL28). Anti-Human MEC (CCL28)-specific antibody was purified by affinity chromatography employing an immobilized Human MEC (CCL28) matrix.
Sandwich ELISA: To detect Human MEC (CCL28) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MEC (CCL28) (500-P297Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MEC (CCL28).
Western Blot: To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor, and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 28; CC chemokine CCL28; CCK 1; CCK1 protein; CCL 28; chemokine (C-C motif) ligand 28 splice variant chi; MEC; Mucosae-associated epithelial chemokine; Protein CCK1; small inducible cytokine A28; small inducible cytokine subfamily A (Cys-Cys), member 28; Small-inducible cytokine A28
Gene Aliases: CCK1; CCL28; MEC; SCYA28
UniProt ID: (Human) Q9NRJ3
Entrez Gene ID: (Human) 56477
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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