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CCL3 Antibody (500-P121-1MG) in WB
Western Blot: To detect Murine MIP-1alpha (CCL3) by Western Blot analysis CCL3 Polyclonal Antibody (Product # 500-P121-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine MIP-1alpha (CCL3) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P121-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse,
Rat
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine MIP-1alpha (CCL3)
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929323
产品详细信息
AA Sequence of recombinant protein: APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-1alpha (CCL3). Anti-Murine MIP-1alpha (CCL3)-specific antibody was purified by affinity chromatography employing an immobilized Murine MIP-1alpha (CCL3) matrix.
Sandwich ELISA: To detect Murine MIP-1alpha (CCL3) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine MIP-1alpha (CCL3) (500-P121Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-1alpha (CCL3).
Western Blot: To detect Murine MIP-1alpha (CCL3) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine MIP-1alpha (CCL3) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C-C motif chemokine 3; H-MIP-1-alpha; Heparin-binding chemotaxis protein; L2G25B; LD78-alpha; Macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1alpha; MIP-1 alpha; MIP-1-alpha; MIP1 (a); mip1 alpha; RP23-320E6.7; SIS-alpha; small inducible cytokine A3; Small-inducible cytokine A3; TY-5
基因别名: AI323804; Ccl3; G0S19-1; LD78alpha; MIP-1alpha; MIP1-(a); MIP1-alpha; Mip1a; Scya3
UniProt ID: (Mouse) P10855
Entrez Gene ID: (Mouse) 20302
chemotaxis
inflammatory response
immune response
positive regulation of cytosolic calcium ion concentration
regulation of cell shape
neutrophil chemotaxis
leukocyte chemotaxis
positive regulation of tumor necrosis factor production
positive regulation of neuron apoptotic process
astrocyte cell migration
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
macrophage chemotaxis
positive regulation of interleukin-1 beta secretion
positive regulation of inflammatory response
cell chemotaxis
