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Invitrogen
CCL4 Monoclonal Antibody (24006)
高级验证
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
图: 1 / 2
CCL4 Antibody (MA5-23713) in ELISA
Nature communications 2020 -
Fig. 1 Ccl5 expression from T cells is repressed by RUNX/CBFbeta complexes. a Expression profiles assessed by ELISA of CCL3, CCL4, and CCL5 and the selected cytokines IL-3, IL-4, and IFNgamma in supernatants of in vitro-stimulated CD4 + and CD8 + T cells at 5 days after stimulation. A summary of three independent measurements on three mice (with their genotypes indicated) are shown. Error bars indicate Mean... View More
产品信息
MA5-23713
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
24006
抗原
Sf 21-derived recombinant human CCL4/MIP-1 beta Ala24-Asn92
偶联物
形式
Lyophilized
浓度
0.5 mg/mL
纯化类型
Protein A/G
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2609459
产品详细信息
In Western blots, this antibody shows less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP-1 alpha and does not cross-react with recombinant mouse (rm) CCL3/MIP-1 alpha, rmCCL4/MIP-1 beta, rhCXCL8/IL-8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
靶标信息
CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ACT-2; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; G-26 T-lymphocyte-secreted protein; HC21; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MGC104418; MGC126025; MGC126026; MIP-1-beta; MIP-1-beta(1-69); MIP1-beta; MIP1beta; PAT 744; Protein H400; RP23-320E6.8; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2
基因别名: ACT2; AT744.1; CCL4; G-26; HC21; LAG-1; LAG1; MIP-1-beta; MIP1B; MIP1B1; SCYA2; SCYA4
UniProt ID: (Human) P13236
Entrez Gene ID: (Human) 6351
monocyte chemotaxis
movement of cell or subcellular component
inflammatory response
immune response
cell adhesion
establishment or maintenance of cell polarity
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
response to virus
response to toxic substance
neutrophil chemotaxis
positive regulation of GTPase activity
negative regulation by host of viral transcription
positive regulation of inflammatory response
positive regulation of calcium-mediated signaling
positive regulation of calcium ion transport
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of natural killer cell chemotaxis
