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CCND1 Antibody (CF803339) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND1 (RC204957, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND1. Positive lysates LY403284 (100 µg) and LC403284 (20 µg) can be purchased separately from OriGene.
产品信息
CF803339
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
OTI8B2
抗原
Full length human recombinant protein of human CCND1 produced in E.coli.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 8% trehalose
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
靶标信息
Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.
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生物信息学
蛋白别名: B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; CCND1 protein; cDNA; FLJ93625; G1/S-specific cyclin-D1; mRNA; PRAD-1; PRAD1 oncogene
基因别名: BCL1; CCND1; D11S287E; PRAD1; U21B31
UniProt ID: (Human) P24385
Entrez Gene ID: (Human) 595
transcription corepressor activity
protein kinase activity
protein binding
transcription factor binding
cyclin-dependent protein serine/threonine kinase regulator activity
enzyme binding
protein kinase binding
protein complex binding
histone deacetylase binding
proline-rich region binding
kinase activator
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
re-entry into mitotic cell cycle
positive regulation of protein phosphorylation
transcription, DNA-templated
protein phosphorylation
cellular response to DNA damage stimulus
lactation
response to iron ion
response to X-ray
response to organonitrogen compound
positive regulation of G2/M transition of mitotic cell cycle
negative regulation of Wnt signaling pathway
negative regulation of epithelial cell differentiation
endoplasmic reticulum unfolded protein response
mitotic G1 DNA damage checkpoint
response to magnesium ion
response to estradiol
response to vitamin E
Leydig cell differentiation
mammary gland epithelial cell proliferation
positive regulation of mammary gland epithelial cell proliferation
response to drug
response to estrogen
response to leptin
fat cell differentiation
response to ethanol
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cell cycle
cell division
response to corticosterone
response to calcium ion
canonical Wnt signaling pathway
mammary gland alveolus development
response to UV-A
negative regulation of cell cycle arrest
liver regeneration
