Invitrogen

CD117 (c-Kit) Monoclonal Antibody (104D2), APC, eBioscience™

CD117 (c-Kit) Antibody in Flow Cytometry (Flow)

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CD117 (c-Kit) Antibody (17-1178-41) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD34 FITC (Product # 11-0349-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD117 (c-Kit) APC (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

17-1178-41

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看10篇已发表文章 10篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

104D2

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2016586

产品详细信息

Description: The 104D2 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells.

Applications Reported: This 104D2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 104D2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

KIT D816V Mast Cells Derived from Induced Pluripotent Stem Cells Recapitulate Systemic Mastocytosis Transcriptional Profile.

International journal of molecular sciences

de Toledo MAS,Fu X,Maié T,Buhl EM,Götz K,Schmitz S,Kaiser A,Boor P,Braunschweig T,Chatain N,Costa IG,Brümmendorf TH,Koschmieder S,Zenke M

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 17-1178-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression.

International journal of molecular sciences

Li N,Wang Y,Wang A,Zhang J,Jia C,Yu C,Song Z,Wang S,Liu L,Yi J,Bao Y,Huang Y,Sun L

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 17-1178-42) in Flow Cytometry

Fri Feb 03 00:00:00 EST 2023

Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR Conductance.

International journal of molecular sciences

Demchenko A,Kondrateva E,Tabakov V,Efremova A,Salikhova D,Bukharova T,Goldshtein D,Balyasin M,Bulatenko N,Amelina E,Lavrov A,Smirnikhina S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 17-1178-42) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2023

A small sustained increase in NOD1 abundance promotes ligand-independent inflammatory and oncogene transcriptional responses.

Science signaling

Rommereim LM,Akhade AS,Dutta B,Hutcheon C,Lounsbury NW,Rostomily CC,Savan R,Fraser IDC,Germain RN,Subramanian N

17-1178-42 was used in Flow Cytometry to provide insight into how a quantitatively small change in protein abundance can produce marked changes in cell state that can serve as the initiator of disease.

Tue Dec 08 00:00:00 EST 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Chemically-Defined, Xeno-Free, Scalable Production of hPSC-Derived Definitive Endoderm Aggregates with Multi-Lineage Differentiation Potential.

Cells

Sahabian A,Sgodda M,Naujok O,Dettmer R,Dahlmann J,Manstein F,Cantz T,Zweigerdt R,Martin U,Olmer R

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 17-1178-42) in Flow Cytometry

Wed Dec 04 00:00:00 EST 2019

IL1RAP potentiates multiple oncogenic signaling pathways in AML.

The Journal of experimental medicine

Mitchell K,Barreyro L,Todorova TI,Taylor SJ,Antony-Debré I,Narayanagari SR,Carvajal LA,Leite J,Piperdi Z,Pendurti G,Mantzaris I,Paietta E,Verma A,Gritsman K,Steidl U

17-1178 was used in Flow cytometry/Cell sorting to show that targeting of interleukin-1 receptor accessory protein via RNA interference, genetic deletion, or antibodies inhibits acute myeloid leukaemia pathogenesis in vitro and in vivo.

Mon Jun 04 00:00:00 EDT 2018

A library of ATTR amyloidosis patient-specific induced pluripotent stem cells for disease modelling and in vitro testing of novel therapeutics.

Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis

Giadone RM,Rosarda JD,Akepati PR,Thomas AC,Boldbaatar B,James MF,Wilson AA,Sanchorawala V,Connors LH,Berk JL,Wiseman RL,Murphy GJ

17-1178-42 was used in Flow Cytometry to characterize six representative iPSC lines from a library of previously reprogrammed iPSC lines and reprogrammable blood samples derived from ATTR amyloidosis patients.

Sat Sep 01 00:00:00 EDT 2018

Human mast cells promote colon cancer growth via bidirectional crosstalk: studies in 2D and 3D coculture models.

Oncoimmunology

Yu Y,Blokhuis B,Derks Y,Kumari S,Garssen J,Redegeld F

17-1178 was used in Flow cytometry/Cell sorting to investigate the impact of primary human mast cells and their mediators on colon cancer growth using 2D and 3D coculture models.

Tue Jan 12 00:00:00 EST 2021

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 17-1178-42) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

生物信息学

蛋白别名: CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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基因别名: C-Kit; CD117; KIT; PBT; SCFR

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UniProt ID: (Human) P10721

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Entrez Gene ID: (Human) 3815

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功能