Invitrogen

CD117 (c-Kit) Monoclonal Antibody (ACK2), Super Bright™ 436, eBioscience™

CD117 (c-Kit) Antibody in Flow Cytometry (Flow)

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CD117 (c-Kit) Antibody (62-1172-82) in Flow

C57BL/6 mouse bone marrow cells were stained with Sca-1 Monoclonal Antibody, PE (Product # 12-5981-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, Super Bright 436 (Product # 62-4031-82) (left) or 0.125 µg of CD117 Monoclonal Antibody, Super Bright 436 (right). Cells in the Lineage low/negative gate were used for analysis.

CD117 (c-Kit) Antibody in Immunocytochemistry (ICC/IF)

CD117 (c-Kit) Antibody in Immunohistochemistry (IHC)

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产品信息

62-1172-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

ACK2

偶联物

Super Bright™ 436 Super Bright™ 436 Super Bright™ 436

激发/发射光谱

413/431 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2744784

产品详细信息

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Applications Reported: This ACK2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Engineered bone marrow as a clinically relevant ex vivo model for primary bone cancer research and drug screening.

Proceedings of the National Academy of Sciences of the United States of America

Griffin KH,Thorpe SW,Sebastian A,Hum NR,Coonan TP,Sagheb IS,Loots GG,Randall RL,Leach JK

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Flow Cytometry

Tue Sep 26 00:00:00 EDT 2023

Landscape of mast cell populations across organs in mice and humans.

The Journal of experimental medicine

Tauber M,Basso L,Martin J,Bostan L,Pinto MM,Thierry GR,Houmadi R,Serhan N,Loste A,Blériot C,Kamphuis JBJ,Grujic M,Kjellén L,Pejler G,Paul C,Dong X,Galli SJ,Reber LL,Ginhoux F,Bajenoff M,Gentek R,Gaudenzio N

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Flow Cytometry

Mon Oct 02 00:00:00 EDT 2023

Morphology of migrating telocytes and their potential role in stem cell differentiation during cartilage development in catfish (Clarias gariepinus).

Microscopy research and technique

Abd-Elhafeez HH,Rutland CS,Soliman SA

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Immunohistochemistry

Fri Sep 01 00:00:00 EDT 2023

Laxative effect of Wenyang Yiqi Decoction on loperamide-induced astriction model mice.

Annals of translational medicine

Su Y,Zhu R,Pang C,He Z,Wu B,Wang X

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Western Blot

Tue Feb 28 00:00:00 EST 2023

Two distinctive types of telocytes in gills of fish: A light, immunohistochemical and ultra-structure study.

Microscopy research and technique

Soliman SA,Sobh A,Ali LA,Abd-Elhafeez HH

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Immunohistochemistry

Tue Nov 01 00:00:00 EDT 2022

The AML-associated K313 mutation enhances C/EBPα activity by leading to C/EBPα overexpression.

Cell death & disease

Gentle IE,Moelter I,Badr MT,Döhner K,Lübbert M,Häcker G

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

The lifespan quantitative trait locus gene Securin controls hematopoietic progenitor cell function.

Haematologica

Brown A,Schuetz D,Han Y,Daria D,Nattamai KJ,Eiwen K,Sakk V,Pospiech J,Saller T,van Zant G,Wagner W,Geiger H

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Flow Cytometry

Tue Apr 27 00:00:00 EDT 2021

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity.

Scientific reports

Abd-Elhafeez HH,Abou-Elhamd AS,Soliman SA

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Immunohistochemistry

Thu Aug 20 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Inhibition of FGF2-Mediated Signaling in GIST-Promising Approach for Overcoming Resistance to Imatinib.

Cancers

Boichuk S,Galembikova A,Mikheeva E,Bikinieva F,Aukhadieva A,Dunaev P,Khalikov D,Petrov S,Kurtasanov R,Valeeva E,Kireev I,Dugina V,Lushnikova A,Novikova M,Kopnin P

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Immunohistochemistry

Wed Jun 24 00:00:00 EDT 2020

Fish telocytes and their relation to rodlet cells in ruby-red-fin shark (rainbow shark) Epalzeorhynchos frenatum (Teleostei: Cyprinidae).

Scientific reports

Abd-Elhafeez HH,Abdo W,Kamal BM,Soliman SA

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Immunohistochemistry

Tue Nov 03 00:00:00 EST 2020

Loss of FGFR3 Delays Acute Myeloid Leukemogenesis by Programming Weakly Pathogenic CD117-Positive Leukemia Stem-Like Cells.

Frontiers in pharmacology

Guo C,Ran Q,Sun C,Zhou T,Yang X,Zhang J,Pang S,Xiao Y

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Hsd3b2 associated in modulating steroid hormone synthesis pathway regulates the differentiation of chicken embryonic stem cells into spermatogonial stem cells.

Journal of cellular biochemistry

Zhang C,Wang M,He N,Ahmed MF,Wang Y,Zhao R,Yu X,Jin J,Song J,Zuo Q,Zhang Y,Li B

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 62-1172-82) in Immunocytochemistry

Mon Jan 01 00:00:00 EST 2018

生物信息学

蛋白别名: belly-spot; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit

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基因别名: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

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UniProt ID: (Mouse) P05532

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Entrez Gene ID: (Mouse) 16590

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功能