Invitrogen

CD11c Monoclonal Antibody (3.9), Biotin, eBioscience™

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CD11c Antibody (13-0116-82) in Flow

Surface staining of normal human peripheral blood cells with Anti-Human CD11c FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

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产品信息

13-0116-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

3.9

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466366

产品详细信息

Description: The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alphaX chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.

Applications Reported: The 3.9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 3.9 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Alveolar macrophages from EVALI patients and e-cigarette users: a story of shifting phenotype.

Respiratory research

Warren KJ,Beck EM,Callahan SJ,Helms MN,Middleton E,Maddock S,Carr JR,Harris D,Blagev DP,Lanspa MJ,Brown SM,Paine R

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Sat Jun 17 00:00:00 EDT 2023

Aluminum hydroxide adjuvant diverts the uptake and trafficking of genetically detoxified pertussis toxin to lysosomes in macrophages.

Molecular microbiology

Jaldin-Fincati J,Moussaoui S,Gimenez MC,Ho CY,Lancaster CE,Botelho R,Ausar F,Brookes R,Terebiznik M

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2022

Upregulation of CD3ζ and L-selectin in antigen-specific cytotoxic T lymphocytes by eliminating myeloid-derived suppressor cells with doxorubicin to improve killing efficacy of neuroblastoma cells in vitro.

Journal of clinical laboratory analysis

Xu W,Li S,Li M,Zhou H,Yang X

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

Isolevuglandins disrupt PU.1-mediated C1q expression and promote autoimmunity and hypertension in systemic lupus erythematosus.

JCI insight

Patrick DM,de la Visitación N,Krishnan J,Chen W,Ormseth MJ,Stein CM,Davies SS,Amarnath V,Crofford LJ,Williams JM,Zhao S,Smart CD,Dikalov S,Dikalova A,Xiao L,Van Beusecum JP,Ao M,Fogo AB,Kirabo A,Harrison DG

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Fri Jul 08 00:00:00 EDT 2022

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

SUMOylation disassembles the tetrameric pyruvate kinase M2 to block myeloid differentiation of leukemia cells.

Cell death & disease

Xia L,Jiang Y,Zhang XH,Wang XR,Wei R,Qin K,Lu Y

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Wed Jan 20 00:00:00 EST 2021

The long non-coding RNA Cancer Susceptibility 15 (CASC15) is induced by isocitrate dehydrogenase (IDH) mutations and maintains an immature phenotype in adult acute myeloid leukemia.

Haematologica

Grasedieck S,Ruess C,Krowiorz K,Lux S,Pochert N,Schwarzer A,Klusmann JH,Jongen-Lavrencic M,Herold T,Bullinger L,Pollack JR,Rouhi A,Kuchenbauer F

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes.

Cell reports

Leach SM,Gibbings SL,Tewari AD,Atif SM,Vestal B,Danhorn T,Janssen WJ,Wager TD,Jakubzick CV

13-0116-82 was used in Flow Cytometry to create a gene expression compendium across 24 distinct human and murine lung and lymph node mononuclear phagocyte (MP), along with human blood and murine spleen MPs, to serve as validation datasets.

Tue Nov 03 00:00:00 EST 2020

Human and Mouse Transcriptome Profiling Identifies Cross-Species Homology in Pulmonary and Lymph Node Mononuclear Phagocytes.

Cell reports

Leach SM,Gibbings SL,Tewari AD,Atif SM,Vestal B,Danhorn T,Janssen WJ,Wager TD,Jakubzick CV

Tue Nov 03 00:00:00 EST 2020

AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.

Oncology letters

Xiu W,Ma J,Lei T,Zhang M

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Pharmacological targeting of miR-155 via the NEDD8-activating enzyme inhibitor MLN4924 (Pevonedistat) in FLT3-ITD acute myeloid leukemia.

Leukemia

Khalife J,Radomska HS,Santhanam R,Huang X,Neviani P,Saultz J,Wang H,Wu YZ,Alachkar H,Anghelina M,Dorrance A,Curfman J,Bloomfield CD,Medeiros BC,Perrotti D,Lee LJ,Lee RJ,Caligiuri MA,Pichiorri F,Croce CM,Garzon R,Guzman ML,Mendler JH,Marcucci G

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD11c monoclonal antibody (Product # 13-0116-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

生物信息学

蛋白别名: CD11 antigen-like family member C; CD11c; complement component 3 receptor 4 subunit; integrin alpha X; Integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); Leu M5; leu M5, alpha subunit; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain

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基因别名: CD11C; ITGAX; SLEB6

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UniProt ID: (Human) P20702

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Entrez Gene ID: (Human) 3687

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功能

receptor activity protein binding metal ion binding integrin

参与通路

cell adhesion integrin-mediated signaling pathway organ morphogenesis extracellular matrix organization heterotypic cell-cell adhesion leukocyte migration positive regulation of protein targeting to mitochondrion