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CD11c Antibody (606-0114-82) in Flow
BALB/c mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82) and 0.25 µg of Armenian Hamster IgG Isotype Control, Alexa Fluor 660 (Product # 606-4888-81) (left) or 0.25 µg of CD11c Monoclonal Antibody, Alexa Fluor 660 (right). Total cells were used for analysis. This data was collected on a 5-laser Cytek Aurora full spectral cytometer.
产品信息
606-0114-82
产品规格
种属反应
Mouse
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
N418
偶联物
激发/发射光谱
663/691 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2896247
产品详细信息
Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.
Applications Reported: This N418 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser
靶标信息
CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD11 antigen-like family member C; CD11c; CD11C (p150) alpha polypeptide; complement receptor 4; Integrin alpha-X; integrin aX; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95
基因别名: AI449405; Cd11c; Cr4; Itgax; N418
UniProt ID: (Mouse) Q9QXH4
Entrez Gene ID: (Mouse) 16411
