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CD138 Antibody (756-1388-82) in IHC (P)
Immunohistochemical analysis of CD138 was performed on formalin-fixed paraffin-embedded human B-cell lymphoma tissue. To expose the target protein, HIER was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted in 1X solution in water in a pressure cooker for 10 minutes, followed by a 5-min cool down and a 5-min wash with ddH2O... View More
产品信息
756-1388-82
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
MI15
抗原
A mixture of U266 and XG-1 human myeloma cell lines
偶联物
Alexa Fluor™ Plus 647
激发/发射光谱
658/675 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
内含物
0.05% sodium azide
保存条件
4° C, store in dark
运输条件
Wet ice
产品详细信息
Description: This MI15 monoclonal antibody reacts with human CD138, also known as syndecan-1. CD138 is a transmembrane protein containing chondroitin sulfate and heparin sulfate moieties responsible for binding to the extracellular matrix components. CD138 is not expressed by mature B cells but is present on pre-B cells and the finally differentiated plasma cells.
Applications Reported: This MI15 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This MI15 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
靶标信息
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD antigen 138; CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; sCD138; soluble CD138; SYND1; syndecan proteoglycan 1; Syndecan-1; Syndecan1
基因别名: CD138; SDC; SDC1; SYND1; syndecan
UniProt ID: (Human) P18827
Entrez Gene ID: (Human) 6382
retinoid metabolic process
ureteric bud development
glycosaminoglycan biosynthetic process
glycosaminoglycan catabolic process
inflammatory response
response to toxic substance
cell migration
glycosaminoglycan metabolic process
wound healing
lipoprotein metabolic process
odontogenesis
response to hydrogen peroxide
myoblast development
response to glucocorticoid
response to cAMP
response to calcium ion
striated muscle cell development
Sertoli cell development
canonical Wnt signaling pathway
positive regulation of exosomal secretion
positive regulation of extracellular exosome assembly
