Invitrogen

CD138 (Syndecan-1) Monoclonal Antibody (DL-101), APC, eBioscience™

CD138 (Syndecan-1) Antibody in Flow Cytometry (Flow)

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CD138 (Syndecan-1) Antibody (17-1389-42) in Flow

Staining of U266 cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD138 (Syndecan-1) APC (purple histogram). Total viable cells were used for analysis.

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产品信息

17-1389-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看7篇已发表文章 7篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

DL-101

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11218892

产品详细信息

Description: The DL-101 monoclonal antibody reacts with human CD138, also known as syndecan-1. CD138 is a transmembrane protein containing chondroitin sulfate and heparin sulfate moieties responsible for binding to the extracellular matrix components. CD138 is not expressed by mature B cells but is present on pre-B cells and the finally differentiated plasma cells.

Applications Reported: This DL-101 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DL-101 antibody has been pre-titrated and tested by flow cytometric analysis of the U266 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Macrophages drive KSHV B cell latency.

Cell reports

Szymula A,Samayoa-Reyes G,Ogolla S,Liu B,Li S,George A,Van Sciver N,Rochford R,Simas JP,Kaye KM

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 17-1389-42) in Flow Cytometry

Tue Jul 25 00:00:00 EDT 2023

Attenuated Dengue virus PV001-DV induces oncolytic tumor cell death and potent immune responses.

Journal of translational medicine

Goldufsky JW,Daniels P,Williams MD,Gupta K,Lyday B,Chen T,Singh G,Kaufman HL,Zloza A,Marzo AL

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 17-1389-42) in Flow Cytometry

Wed Jul 19 00:00:00 EDT 2023

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

PLoS pathogens

Akidil E,Albanese M,Buschle A,Ruhle A,Pich D,Keppler OT,Hammerschmidt W

17-1389-42 was used in Flow Cytometry to enable efficient targeting of specific gene loci in quiescent human B cells supporting basic research as well as immunotherapeutic strategies.

Thu Apr 01 00:00:00 EDT 2021

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

PLoS pathogens

Akidil E,Albanese M,Buschle A,Ruhle A,Pich D,Keppler OT,Hammerschmidt W

17-1389-42 was used in Flow Cytometry to enable efficient targeting of specific gene loci in quiescent human B cells supporting basic research as well as immunotherapeutic strategies.

Thu Apr 01 00:00:00 EDT 2021

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD138 (Syndecan-1) monoclonal antibody (Product # 17-1389-42) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Mitochondrial-Targeted Decyl-Triphenylphosphonium Enhances 2-Deoxy-D-Glucose Mediated Oxidative Stress and Clonogenic Killing of Multiple Myeloma Cells.

PloS one

Schibler J,Tomanek-Chalkley AM,Reedy JL,Zhan F,Spitz DR,Schultz MK,Goel A

17-1389 was used in Flow cytometry/Cell sorting to study how mitochondrial-targeted decyl-triphenylphosphonium enhances 2-deoxy-d-glucose mediated oxidative stress and clonogenic killing of multiple myeloma cells.

Thu Jul 06 00:00:00 EDT 2017

Mitochondrial-Targeted Decyl-Triphenylphosphonium Enhances 2-Deoxy-D-Glucose Mediated Oxidative Stress and Clonogenic Killing of Multiple Myeloma Cells.

PloS one

Schibler J,Tomanek-Chalkley AM,Reedy JL,Zhan F,Spitz DR,Schultz MK,Goel A

Thu Jul 06 00:00:00 EDT 2017

PPARγ negatively regulates T cell activation to prevent follicular helper T cells and germinal center formation.

PloS one

Park HJ,Kim DH,Choi JY,Kim WJ,Kim JY,Senejani AG,Hwang SS,Kim LK,Tobiasova Z,Lee GR,Craft J,Bothwell AL,Choi JM

17-1389 was used in Flow cytometry/Cell sorting to suggest that PPARγ has a regulatory role for follicular helper T cells and germinal center reaction to prevent autoimmunity.

Wed Oct 07 00:00:00 EDT 2015

生物信息学

蛋白别名: CD antigen 138; CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; sCD138; soluble CD138; SYND1; syndecan proteoglycan 1; Syndecan-1; Syndecan1

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基因别名: CD138; SDC; SDC1; SYND1; syndecan

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UniProt ID: (Human) P18827

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Entrez Gene ID: (Human) 6382

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功能

glycoprotein binding protein binding protein C-terminus binding transmembrane signal receptor

参与通路