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CD14 Antibody (PA5-78957) in IHC (P)
Immunohistochemistry analysis of CD14 on paraffin-embedded rat lymphaden tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with CD14 polyclonal antibody (Product# PA5-78957) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Developm... View More
产品信息
PA5-78957
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived mouse CD14 recombinant protein (Position: S16-A260).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2746073
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD 14; CD14; CD14 antigen; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14
基因别名: Cd14
UniProt ID: (Rat) Q63691, (Mouse) P10810
Entrez Gene ID: (Rat) 60350, (Mouse) 12475
response to molecule of bacterial origin
inflammatory response
response to heat
response to magnesium ion
response to lipopolysaccharide
positive regulation of tumor necrosis factor production
response to tumor necrosis factor
innate immune response
response to ethanol
positive regulation of endocytosis
positive regulation of cytokine secretion
response to electrical stimulus
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
immune system process
