Invitrogen

CD14 Monoclonal Antibody (5A3)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1个已发表图片

2 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

CD14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD14 Antibody (MA5-14773) in IHC (P)

Immunohistochemical analysis of CD14 was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Foll... View More

CD14 Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))

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CD14 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD14 was observed in THP-1 differentiated to M1 macrophage and THP-1 differentiated to M1 macrophage followed by lipopolysaccharide treatment and not in Jurkat, Raji and MOLT-4 which are reported to be negative using Anti-CD14 Monoclonal Antibody (5A3) (Product # MA5-14773) in western blot. {RE}

产品信息

MA5-14773

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

5A3

抗原

Purified recombinant fragment of human CD14 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10983091

产品详细信息

MA5-14773 targets CD14 in indirect ELISA, FACS and IHC applications and shows reactivity with Human samples.

The MA5-14773 immunogen is purified recombinant fragment of human CD14 expressed in E. Coli.

靶标信息

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Establishment of Human Leukocyte Antigen-Mismatched Immune Responses after Transplantation of Human Liver Bud in Humanized Mouse Models.

Cells

Mori A,Murata S,Tashiro N,Tadokoro T,Okamoto S,Otsuka R,Wada H,Murata T,Takahashi T,Seino KI,Taniguchi H

Published figure using CD14 monoclonal antibody (Product # MA5-14773) in Immunohistochemistry (PFA fixed)

Tue Feb 23 00:00:00 EST 2021

Establishment of Human Leukocyte Antigen-Mismatched Immune Responses after Transplantation of Human Liver Bud in Humanized Mouse Models.

Cells

Mori A,Murata S,Tashiro N,Tadokoro T,Okamoto S,Otsuka R,Wada H,Murata T,Takahashi T,Seino KI,Taniguchi H

Published figure using CD14 monoclonal antibody (Product # MA5-14773) in Immunohistochemistry (PFA fixed)

Tue Feb 23 00:00:00 EST 2021

Establishment of Human Leukocyte Antigen-Mismatched Immune Responses after Transplantation of Human Liver Bud in Humanized Mouse Models.

Cells

Mori A,Murata S,Tashiro N,Tadokoro T,Okamoto S,Otsuka R,Wada H,Murata T,Takahashi T,Seino KI,Taniguchi H

Published figure using CD14 monoclonal antibody (Product # MA5-14773) in Immunohistochemistry (PFA fixed)

Tue Feb 23 00:00:00 EST 2021

生物信息学

蛋白别名: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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基因别名: CD14

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UniProt ID: (Human) P08571

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Entrez Gene ID: (Human) 929

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功能

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

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