Invitrogen

CD14 Monoclonal Antibody (TuK4), PE-Cyanine5.5

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CD14 Antibody (MHCD1418) in Flow

Human peripheral blood monocytes stained using PE-Cy®5.5 of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.

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产品信息

MHCD1418

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

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产品规格

种属反应

Human

已发表种属

Bovine, Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

TuK4

抗原

Human CD14

偶联物

PE-Cyanine5.5 PE-Cyanine5.5 PE-Cyanine5.5

激发/发射光谱

564/692 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with 10% sucrose, BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10371748

产品详细信息

The R-phycoerythrin (PE)-Cy5.5® tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

靶标信息

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

PD-1 directed immunotherapy alters Tfh and humoral immune responses to seasonal influenza vaccine.

Nature immunology

Herati RS,Knorr DA,Vella LA,Silva LV,Chilukuri L,Apostolidis SA,Huang AC,Muselman A,Manne S,Kuthuru O,Staupe RP,Adamski SA,Kannan S,Kurupati RK,Ertl HCJ,Wong JL,Bournazos S,McGettigan S,Schuchter LM,Kotecha RR,Funt SA,Voss MH,Motzer RJ,Lee CH,Bajorin DF,Mitchell TC,Ravetch JV,Wherry EJ

MHCD1418 was used in Flow cytometry/Cell sorting to examine the impact of anti-PD-1 immunotherapy on the Tfh-B cell axis responding to unrelated viral antigens.

Mon Aug 01 00:00:00 EDT 2022

Transcriptional, epigenetic and metabolic signatures in cardiometabolic syndrome defined by extreme phenotypes.

Clinical epigenetics

Seyres D,Cabassi A,Lambourne JJ,Burden F,Farrow S,McKinney H,Batista J,Kempster C,Pietzner M,Slingsby O,Cao TH,Quinn PA,Stefanucci L,Sims MC,Rehnstrom K,Adams CL,Frary A,Ergüener B,Kreuzhuber R,Mocciaro G,D'Amore S,Koulman A,Grassi L,Griffin JL,Ng LL,Park A,Savage DB,Langenberg C,Bock C,Downes K,Wareham NJ,Allison M,Vacca M,Kirk PDW,Frontini M

Published figure using CD14 monoclonal antibody (Product # MHCD1418) in Flow Cytometry

Sat Mar 12 00:00:00 EST 2022

Transcriptional, epigenetic and metabolic signatures in cardiometabolic syndrome defined by extreme phenotypes.

Clinical epigenetics

Published figure using CD14 monoclonal antibody (Product # MHCD1418) in Flow Cytometry

Sat Mar 12 00:00:00 EST 2022

Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition.

Cell

Pasciuto E,Burton OT,Roca CP,Lagou V,Rajan WD,Theys T,Mancuso R,Tito RY,Kouser L,Callaerts-Vegh Z,de la Fuente AG,Prezzemolo T,Mascali LG,Brajic A,Whyte CE,Yshii L,Martinez-Muriana A,Naughton M,Young A,Moudra A,Lemaitre P,Poovathingal S,Raes J,De Strooper B,Fitzgerald DC,Dooley J,Liston A

MHCD1418 was used in Flow cytometry/Cell sorting to investigate the function of brain-resident CD4 T cells.

Thu Aug 06 00:00:00 EDT 2020

Novel Markers of Angiogenesis in the Setting of Cognitive Impairment and Dementia.

Journal of Alzheimer's disease : JAD

Callahan CM,Apostolova LG,Gao S,Risacher SL,Case J,Saykin AJ,Lane KA,Swinford CG,Yoder MC

MHCD1418 was used in Flow cytometry/Cell sorting to explore the relationship between angiogenesis activity and evidence of neurodegeneration among older adults.

Mon May 10 00:00:00 EDT 2021

Differential transcriptome and development of human peripheral plasma cell subsets.

JCI insight

Garimalla S,Nguyen DC,Halliley JL,Tipton C,Rosenberg AF,Fucile CF,Saney CL,Kyu S,Kaminski D,Qian Y,Scheuermann RH,Gibson G,Sanz I,Lee FE

MHCD1418 was used in Flow cytometry/Cell sorting to study the heterogeneity of human antibody secreting cells after vaccination.

Thu May 02 00:00:00 EDT 2019

Effects of treatment of preweaning dairy calves with recombinant bovine somatotropin on immune responses and somatotropic axis.

Journal of dairy science

Belli AL,Reis RB,Veronese A,Moreira R,Flanagan K,Driver J,Nelson CD,Clapper JA,Ballou MA,Jeong KC,Chebel RC

MHCD1418 was used in Flow cytometry/Cell sorting to investigate the effects of treatment of pre-weaning calves with recombinant bovine somatotropin on the somatotropic axis, selected immune parameters, and hematology of calves around weaning.

Sun Jul 01 00:00:00 EDT 2018

生物信息学

蛋白别名: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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基因别名: CD14

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UniProt ID: (Human) P08571

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Entrez Gene ID: (Human) 929

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功能

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

参与通路