Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 3
CD14 (soluble) Antibody (500-P320-1MG) in WB
Western Blot: To detect Human sCD14 by Western Blot analysis CD14 (soluble) Polyclonal Antibody (Product # 500-P320-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sCD14 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P320-1MG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
HEK293 cells-derived Recombinant Human sCD14
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930390
产品详细信息
AA Sequence of recombinant protein: TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sCD14. Anti-Human sCD14-specific antibody was purified by affinity chromatography employing an immobilized Human sCD14 matrix.
Sandwich ELISA: To detect Human sCD14 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sCD14 (500-P320Bt) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human sCD14.
Western Blot: To detect Human sCD14 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sCD14 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14
基因别名: CD14
UniProt ID: (Human) P08571
Entrez Gene ID: (Human) 929
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
receptor-mediated endocytosis
phagocytosis
apoptotic process
inflammatory response
cell surface receptor signaling pathway
I-kappaB kinase/NF-kappaB signaling
response to heat
lipopolysaccharide-mediated signaling pathway
response to magnesium ion
positive regulation of type I interferon production
positive regulation of interferon-gamma production
positive regulation of tumor necrosis factor production
negative regulation of MyD88-independent toll-like receptor signaling pathway
toll-like receptor 4 signaling pathway
response to tumor necrosis factor
TRIF-dependent toll-like receptor signaling pathway
toll-like receptor TLR1:TLR2 signaling pathway
toll-like receptor TLR6:TLR2 signaling pathway
innate immune response
response to ethanol
positive regulation of endocytosis
positive regulation of cytokine secretion
response to electrical stimulus
necroptotic process
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to diacyl bacterial lipopeptide
cellular response to triacyl bacterial lipopeptide
apoptotic signaling pathway
