Invitrogen

CD140b (PDGFRB) Monoclonal Antibody (APB5), PE-eFluor™ 610, eBioscience™

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产品信息

61-1402-82

应用
建议稀释比
已发表文章

免疫组化 (IHC)

-
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免疫细胞化学 (ICC/IF)

-
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流式细胞分析 (Flow)

0.5 µg/test
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产品规格

种属反应

Mouse

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2a, kappa

推荐的同型对照

Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

APB5

偶联物

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

激发/发射光谱

565/606 nm 查看光谱 spectra

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2815310

产品详细信息

Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.

Applications Reported: This APB5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

靶标信息

PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

生物信息学

蛋白别名: Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b; CD140b antigen; PDGF beta chain; PDGF Receptor beta; PDGF-R-beta; PDGFR-1; PDGFR-beta; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1

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基因别名: AI528809; CD140b; Pdgfr; PDGFR-1; Pdgfr1; Pdgfrb

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UniProt ID: (Mouse) P05622

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Entrez Gene ID: (Mouse) 18596

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功能
nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity signal transducer activity platelet-derived growth factor-activated receptor activity platelet-derived growth factor beta-receptor activity receptor binding platelet-derived growth factor receptor binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding vascular endothelial growth factor binding phosphatidylinositol 3-kinase binding platelet-derived growth factor binding transmembrane signal receptor
参与通路
in utero embryonic development kidney development tissue homeostasis glycosaminoglycan biosynthetic process protein phosphorylation nitrogen compound metabolic process chemotaxis signal transduction transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development positive regulation of cell proliferation positive regulation of phospholipase C activity positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration phosphorylation cell migration peptidyl-tyrosine phosphorylation hemopoiesis adrenal gland development positive regulation of cell migration positive regulation of phosphoprotein phosphatase activity regulation of actin cytoskeleton organization positive regulation of collagen biosynthetic process cell migration involved in vasculogenesis intracellular signal transduction metanephric mesenchymal cell migration platelet-derived growth factor receptor-beta signaling pathway positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway aorta morphogenesis cellular response to platelet-derived growth factor stimulus positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway negative regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity positive regulation of mitotic nuclear division phosphatidylinositol metabolic process protein autophosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of smooth muscle cell proliferation skeletal system morphogenesis smooth muscle tissue development regulation of peptidyl-tyrosine phosphorylation positive regulation of chemotaxis cardiac myofibril assembly cell chemotaxis cardiac vascular smooth muscle cell differentiation cell migration involved in coronary angiogenesis retina vasculature development in camera-type eye positive regulation of ERK1 and ERK2 cascade smooth muscle cell chemotaxis metanephric glomerular mesangial cell proliferation involved in metanephros development metanephric glomerular capillary formation positive regulation of calcium ion import positive regulation of reactive oxygen species metabolic process positive regulation of DNA biosynthetic process
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