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CD16-2 Antibody (MA5-29709) in ICC/IF
Immunofluorescence staining of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with10% serum, and incubated with CD16-2 Recombinant Rabbit Monoclonal Antibody (12) (Product # MA5-29709, 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
产品信息
MA5-29709
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
012
抗原
Recombinant Mouse FCGR4 protein (Met1-Gln203)
偶联物
Unconjugated
形式
Liquid
浓度
1.18 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2785533
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Mouse CD16-2/FCGR4.
靶标信息
FcgRIV, also known as CD16-2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16-2 is the mouse homolog of human FcgRIIIA. CD16-2 is a low-affinity IgE receptor for all allotypes and the ligation of FcgRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD16-2; CD16a; Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc gammaRIV; Fc receptor-like 3; FcgammaRIV; IgG Fc receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-A
基因别名: 4833442P21Rik; CD16-2; FcgammaRIV; Fcgr3a; Fcgr4; FcgRIV; Fcrl3
UniProt ID: (Mouse) A0A0B4J1G0
Entrez Gene ID: (Mouse) 246256
