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Invitrogen
CD16/CD32 Monoclonal Antibody (93), Super Bright™ 436, eBioscience™
图: 1 / 1
CD16/CD32 Antibody (62-0161-82) in Flow
C57BL/6 mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0454-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321-82) (left) or 0.25 µg of CD16/32 Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
62-0161-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Rat
/ IgG2a, lambda
分类
Monoclonal
类型
Antibody
克隆号
93
偶联物
激发/发射光谱
413/431 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2734888
产品详细信息
Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.
Applications Reported: This 93 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
靶标信息
CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD16A; CD16B; CD32; CD32 receptor 2; Fc gamma receptor IIB; Fc gamma receptor III; Fc gamma RIIB; Fc-gamma RII; fc-gamma RIII; Fc-gamma-RII; fc-gamma-RIIB; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; IgG Fc receptor II beta; igG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Ly-17; Lymphocyte antigen 17; RP11-5K23.1
基因别名: AI528646; CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1
UniProt ID: (Mouse) P08101
Entrez Gene ID: (Mouse) 14130, (Mouse) 14131
negative regulation of type I hypersensitivity
immune complex clearance
negative regulation of immunoglobulin production
negative regulation of inflammatory response to antigenic stimulus
negative regulation of acute inflammatory response to antigenic stimulus
negative regulation of humoral immune response mediated by circulating immunoglobulin
receptor-mediated endocytosis
phagocytosis, engulfment
defense response
immune response
cell surface receptor signaling pathway
response to bacterium
immunoglobulin mediated immune response
antigen processing and presentation of exogenous peptide antigen via MHC class II
negative regulation of B cell proliferation
endocytic recycling
negative regulation of interleukin-10 production
negative regulation of neutrophil apoptotic process
intracellular signal transduction
mast cell activation
negative regulation of phagocytosis
positive regulation of phagocytosis
negative regulation of immune response
cellular response to molecule of bacterial origin
antibody-dependent cellular cytotoxicity
positive regulation of type IIa hypersensitivity
positive regulation of type III hypersensitivity
positive regulation of type I hypersensitivity
serotonin secretion
phagocytosis, recognition
neutrophil chemotaxis
Fc-gamma receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
antigen processing and presentation of exogenous peptide antigen via MHC class I
regulation of immune response
