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Invitrogen
CD160 Monoclonal Antibody (eBioCNX46-3 (CNX46-3)), eBioscience™
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CD160 Antibody (14-1601-81) in Flow
C57BL/6 splenocytes were stained with 0.5 µg of Anti-Mouse CD160 Purified (Product # 14-1601-81) followed by F (ab')2 Anti-Rat IgG PE (Product # 12-4012) and subsequently with Anti-Mouse CD3e FITC (Product # 11-0031-82) and Anti-Mouse NK1-1 APC (Product # 17-5941-82). The histogram (right) demonstrates staining of Anti-Mouse CD160 Purified on NK1-1+CD3. cells (red histogram and NK1-1+CD3+ cells (green histogram), as gated in the dot plot (left).
产品信息
14-1601-81
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBioCNX46-3 (CNX46-3)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1210783
产品详细信息
Description: CD160 is a glycosylphosphatidylinositol (GPI)-anchored Ig-like glycoprotein first identified on human lymphocytes with the monoclonal antibody BY55. In mice, CD160 is expressed on almost all (intestinal intraepithelial lymphocytes) iIELs, NKT cells, most TCRgammadelta T cells, few NK cells and a minor subset of CD8+ T cells. Murine CD160 has been shown to bind to a wide range of classical and non classical MHC class I molecules and regulate NK cell activation. In vitro, CD3 activation of murine CD8+ T cells increases the expression of CD160 and induces the release of soluble CD160 (sCD160). In human, CD160 mAb cross-linking triggers TNF alpha, IFN gamma and IL-6 cytokine production by peripheral blood NK cells and inhibits tube formation and induces apoptosis of endothelial cells. In mice, cross-linking of CD160 with the CNX46-3 antibody regulates NK cell activation both positively and negatively, depending on the stimulus.
Applications Reported: This eBioCNX46-3 (CNX46-3) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). (Please use Functional Grade purified eBioCNX46-3 (CNX46-3), Product # 16-1601, in functional assays).
Applications Tested: This eBioCNX46-3 (CNX46-3) antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD160 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. It is involved in cell proliferation, cell surface receptor signaling pathways, cellular defense response, and regulation of immune response.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD160; CD160 antigen; natural killer cell BY55; natural killer cell receptor; Natural killer cell receptor BY55
基因别名: AU045688; By55; Cd160
UniProt ID: (Mouse) O88875
Entrez Gene ID: (Mouse) 54215
