Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD162 (PSGL-1) Monoclonal Antibody (4RA10), Alexa Fluor™ 647, eBioscience™
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
图: 1 / 3
CD162 (PSGL-1) Antibody (51-1621-82) in Flow
BALB/c mouse splenocytes were stained with CD3 Monoclonal Antibody, PE (Product # 12-0031-82) and 0.03 µg of Rat IgG1 kappa Isotype Control, Alexa Fluor 647 (Product # 51-4301-81) (left) or 0.03 µg of CD162 (PSGL-1) Monoclonal Antibody, Alexa Fluor 647 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
51-1621-82
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
4RA10
抗原
Mouse PSGL-1 human IgG1 fusion protein
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2848402
产品详细信息
Description: This 4RA10 monoclonal antibody reacts with mouse CD162, which is also known as P-selectin glycoprotein ligand-1 (PSGL-1). This clone has been successfully used in flow cytometry and has been reported to block binding of CD162 to CD62P and CD62L.
Applications Reported: This 4RA10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 4RA10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
靶标信息
CD162 (PSGL-1) is a 120 kDa homodimer protein found on the surface of leukocytes and platelets. CD162 is known to bind CD62P (P-selectin), CD62L (L-selectin), and to a lower degree CD62E (E-selectin). These interactions play a critical role in leukocyte adhesion, rolling and extravasation. CD162 has been shown to be an important immune marker of T cell exhaustion in chronic viral infections and cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Cutaneous lymphocyte associated antigen; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand-1; selectin P ligand; SELPG
基因别名: CD162; Psgl-1; Psgl1; Selp1; Selpl
Entrez Gene ID: (Mouse) 20345
