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Application
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CD166 (ALCAM) Antibody (12-1668-42) in Flow
Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD166 (ALCAM) PE (purple histogram). Cells in the monocyte gate were used for analysis.
产品信息
12-1668-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
3A6
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572587
产品详细信息
Description: This 3A6 monoclonal antibody reacts with human CD166, which is also known as activated leukocyte cell adhesion molecule (ALCAM). CD166 is an immunoglobulin superfamily cell adhesion molecule that is broadly expressed on developing and adult hematopoietic (e.g., activated T and B cells, monocytes, and dendritic cells) and non-hematopoietic (e.g., neural, endothelial, and epithelial) tissues. CD166 has also been detected in melanoma, colorectal carcinoma, as well as cancers of the breast, prostate, and bladder. CD166 mediates cell-cell adhesion by forming low-affinity homophilic interactions (ALCAM-ALCAM) or high-affinity heterophilic interactions with its ligand CD6 (ALCAM-CD6). CD166 plays a role in development, inflammation, and neutrophil migration. Moreover, its expression can be used as a prognostic marker for several cancers and has been reported to be a cancer stem cell marker.
The 3A6 antibody has been reported to induce the internalization of CD166 and its recycling back to the cell surface.
Applications Reported: This 3A6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3A6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Activated leukocyte cell adhesion molecule; CD166; CD166 antigen; FLJ38514; MGC71733
基因别名: ALCAM; CD166; MEMD
UniProt ID: (Human) Q13740
Entrez Gene ID: (Human) 214
