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Invitrogen

CD171 Monoclonal Antibody (UJ127), Biotin

高级验证

11 篇参考文献

查看其他43个CD171 (L1CAM)抗体

说明书

实验方案

技术支持

货号 MA5-14137

500 µL

说明书

实验方案

技术支持

抗体检测数据 (6)
高级验证 (2)

Application

图: 1 / 8

CD171 Antibody (MA5-14137) in WB

Western blot was performed using Anti-CD171 Monoclonal Antibody (UJ127), Biotin(Product # MA5-14137) and a 250, 85kDa band corresponding to CD171 was observed across the panel tested except Caco-2 which is reported to be negative. Whole cell extracts (30 µg lysate) of SW480 (Lane 1), Caco-2 (Lane 2), PANC-1 (Lane 3) and U-2 OS (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System... View More

CD171 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD171 Antibody in Immunoprecipitation (IP)

Product Image Gallery

CD171 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD171 was observed in in SW480, PANC-1 and U-2 OS in comparison to Caco-2 using Anti-CD171 Monoclonal Antibody (UJ127), Biotin (Product # MA5-14137) in Western Blot. {RE}

产品信息

MA5-14137

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100

流式细胞分析 (Flow)

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

2 µg/mL

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

UJ127

抗原

Homogenous suspension of 16 week human fetal brain

查看免疫原

偶联物

Biotin

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10990347

产品详细信息

MA5-14137 targets L1 Cell Adhesion Molecule in IHC (P), IP, and WB applications and shows reactivity with Human samples.

The MA5-14137 immunogen is homogenous suspension of 16 week human fetal brain.

靶标信息

L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Targeting L1 cell adhesion molecule expression using liposome-encapsulated siRNA suppresses prostate cancer bone metastasis and growth.

Oncotarget

Sung SY,Wu IH,Chuang PH,Petros JA,Wu HC,Zeng HJ,Huang WC,Chung LW,Hsieh CL

MA5-14137 was used in ELISA to determine how using liposome-encapsulated siRNA to target L1 cell adhesion molecule expression suppresses prostate cancer bone metastasis and growth

Thu Oct 30 00:00:00 UTC 2014

Adhesion molecule L1 is down-regulated in malignant peripheral nerve sheath tumors versus benign neurofibromatosis type 1-associated tumors.

Oral surgery, oral medicine, oral pathology and oral radiology

Blessmann M,Gröbe A,Quaas A,Kaifi JT,Mistakidis G,Bernreuther C,Sauter G,Gros S,Rawnaq T,Friedrich R,Mautner VF,Smeets R,Heiland M,Schachner M,Izbicki JR

MA5-14137 was used in immunohistochemistry to study the expression of the L1 adhesion molecule in malignant peripheral nerve sheath tumors and benign neurofibromatosis type 1-associated tumors

Wed Feb 01 00:00:00 UTC 2012

Two alcohol binding residues interact across a domain interface of the L1 neural cell adhesion molecule and regulate cell adhesion.

The Journal of biological chemistry

Dou X,Menkari CE,Shanmugasundararaj S,Miller KW,Charness ME

MA5-14137 was used in flow cytometry to characterize two alcohol binding residues in L1 neural cell adhesion molecule and their involvement in cell adhesion

Fri May 06 00:00:00 UTC 2011

DOG1 and CD117 are the antibodies of choice in the diagnosis of gastrointestinal stromal tumours.

Histopathology

Novelli M,Rossi S,Rodriguez-Justo M,Taniere P,Seddon B,Toffolatti L,Sartor C,Hogendoorn PC,Sciot R,Van Glabbeke M,Verweij J,Blay JY,Hohenberger P,Flanagan A,Dei Tos AP

MA5-14137 was used in immunohistochemistry to study the value of COG1 and CD117 immunohistochemistry in the diagnosis of gastrointestinal stromal tumors

Sun Aug 01 00:00:00 UTC 2010

Subset of esophageal adenocarcinoma expresses adhesion molecule l1 in contrast to squamous cell carcinoma.

Anticancer research

Rawnaq T,Kleinhans H,Uto M,Schurr PG,Reichelt U,Cataldegirmen G,Gawad KA,Yekebas EF,Schachner M,Izbicki JR,Kaifi JT

MA5-14137 was used in immunohistochemistry to study neural cell adhesion molecule L1 overexpression in a subgroup of esophageal cancer

Wed Apr 01 00:00:00 UTC 2009

L1 is associated with micrometastatic spread and poor outcome in colorectal cancer.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Kaifi JT,Reichelt U,Quaas A,Schurr PG,Wachowiak R,Yekebas EF,Strate T,Schneider C,Pantel K,Schachner M,Sauter G,Izbicki JR

MA5-14137 was used in immunohistochemistry to study the role of the L1 adhesion molecule in micrometastatic spread and poor outcome in colorectal cancer

Thu Nov 01 00:00:00 UTC 2007

L1 is associated with favorable outcome in neuroblastomas in contrast to adult tumors.

Annals of surgical oncology

Wachowiak R,Fiegel HC,Kaifi JT,Quaas A,Krickhahn A,Schurr PG,Erttmann R,Schachner M,Kluth D,Sauter G,Izbicki JR

MA5-14137 was used in immunohistochemistry to study the association of the L1 neuronal adhesion molecule with favorable outcome in childhood neuroblastomas

Sat Dec 01 00:00:00 UTC 2007

L1 (CD171) is highly expressed in gastrointestinal stromal tumors.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Kaifi JT,Strelow A,Schurr PG,Reichelt U,Yekebas EF,Wachowiak R,Quaas A,Strate T,Schaefer H,Sauter G,Schachner M,Izbicki JR

MA5-14137 was used in immunohistochemistry to study the expression of L1 (CD171) in gastrointestinal stromal tumors

Wed Mar 01 00:00:00 UTC 2006

Characterization of gene expression in mucinous cystic neoplasms of the pancreas using oligonucleotide microarrays.

Oncogene

Fukushima N,Sato N,Prasad N,Leach SD,Hruban RH,Goggins M

MA5-14137 was used in immunohistochemistry to investigate the changes of gene expression in pancreatic mucinous cystic neoplasms

Thu Dec 02 00:00:00 UTC 2004

Identification of cell surface and secreted proteins essential for tumor cell survival using a genetic suppressor element screen.

Oncogene

Gelman MS,Ye XK,Stull R,Suhy D,Jin L,Ng D,Than B,Ji M,Pan A,Perez P,Sun Y,Yeung P,Garcia LM,Harte R,Lu Y,Lamar E,Tavassoli R,Kennedy S,Osborn S,Chin DJ,Meshaw K,Holzmayer TA,Axenovich SA,Abo A

MA5-14137 was used in flow cytometry to identify molecular markers and therapeutic targets for tumors

Thu Oct 21 00:00:00 UTC 2004

Patterning axonal guidance molecules using a novel strategy for microcontact printing.

Neurochemical research

Oliva AA,James CD,Kingman CE,Craighead HG,Banker GA

MA5-14137 was used in western blot to develop a protocol for microprinting proteins on a substrate in order to control neurite growth in culture

Sat Nov 01 00:00:00 UTC 2003

生物信息学

蛋白别名: antigen identified by monoclonal antibody R1; CD 171; CD171; CD171 molecule; N-CAM-L1; Neural cell adhesion molecule L1; sCD171; sL1 CAM; sL1CAM; soluble CD 171; soluble CD171; soluble L1 CAM; soluble L1CAM

基因别名: CAML1; CD171; HSAS; HSAS1; L1CAM; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1

UniProt ID: (Human) P32004

Entrez Gene ID: (Human) 3897

参与通路

chemotaxis cell adhesion nervous system development axon guidance positive regulation of axon extension leukocyte migration

CD171 Monoclonal Antibody (UJ127), Biotin

CD171 Antibody (MA5-14137) in WB

产品信息

MA5-14137

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

CD171 Monoclonal Antibody (UJ127), Biotin

CD171 Antibody (MA5-14137) in WB

产品信息

MA5-14137

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID